The propagation mechanism of Semaphorin3A signaling in neurons

2011 Fiscal Year Final Research Report

• Project/Area Number: 21700411
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Yokohama City University
• Principal Investigator: YAMASHITA Naoya 横浜市立大学, 医学部, 助教 (40508793)
• Research Collaborator: YAMANE Masayuki 横浜市立大学, 医学研究科, 大学院生
• Project Period (FY): 2009 – 2011
• Keywords: セマフォリン / 神経栄養因子 / シグナル伝播

Research Abstract

Neurons are highly polarized cells with axon, dendrites, and cell body. These subcellular compartments need to communicate with each other. For example, signals received in distal axon travel long distances to reach the cell body and/or dendrites. In present study, I identified novel mechanism that the signal elicited by axon guidance molecule which is received at axonal growth cone, is transmitted towards cell body and dendrites. I further found that this novel signaling mediates dendritic morphology and maturation. Therefore I propose the model that the axon guidance signal which is received at distal axon simultaneously regulates dendritic development by this novel signal propagation mechanism.

Research Products

• [Journal Article] Semaphorin3A facilitates axonal transport through a local calcium signaling and tetrodotoxin-sensitive voltage-gated sodium channels (2012)
  • Author(s): Yamane M, Yamashita N, Yamamoto H, Iizuka A, Shouji M, Usui H, Goshima Y.
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 422(2) Pages: 333-38
  • DOI: DOI:10.1016/j.bbrc.2012.05.003
• [Journal Article] Collapsin Response Mediator Proteins Regulate Neuronal Development and Plasticity by Switching Their Phosphorylation Status (2012)
  • Author(s): Yamashita N, Goshima Y.
  • Journal Title: Molecular Neurobiology Volume: 45(2) Pages: 234-46
  • DOI: DOI:10.1007/s12035-012-8242-4
• [Journal Article] Phosphorylation of CRMP2(Collapsin Response Mediator Protein 2) Is Involved in Proper Dendritic Field Organization (2011)
  • Author(s): Yamashita N, Ohshima T, Nakamura F, Kolattukudy P, Honnorat J, Mikoshiba K, Goshima Y.
  • Journal Title: Journal of Neuroscience Volume: 32(4) Pages: 1360-65
  • DOI: DOI:10.1523/JNEUROSCI.5563-11.2012
• [Journal Article] CRMP5(collapsin response mediator protein 5) regulates dendritic development and synaptic plasticity in the cerebellar Purkinje cells (2011)
  • Author(s): Yamashita N, Mosinger B, Roy A, Miyazaki M, Ugajin K, Nakamura F, Sasaki Y, Yamaguchi K, Kolattukudy P, Goshima Y.
  • Journal Title: Journal of Neuroscience Volume: 31(5) Pages: 1773-79
  • DOI: DOI:10.1523/JNEUROSCI.5337-10.2011
• [Journal Article] Semaphorin 3A induces CaV2.3 channel-dependent conversion of axons to dendrites (2011)
  • Author(s): Nishiyama M, Togashi K, von Schimmelmann MJ, Lim CS, Maeda S, Yamashita N, Goshima Y, Ishii S, Hong K.
  • Journal Title: Nature Neuroscience Volume: 13(6) Pages: 676-85
  • DOI: DOI:10.1038/ncb2255
• [Journal Article] Structural basis for semaphorin signalling through the plexin receptor (2010)
  • Author(s): Nogi T, Yasui N, Mihara E, Matsunaga Y, Noda M, Yamashita N, Toyofuku T, Uchiyama S, Goshima Y, Kumanogoh A, Takagi J.
  • Journal Title: Nature Volume: 467(7319) Pages: 1123-7
  • DOI: DOI:10.1038/nature09473
• [Journal Article] Computational analysis of the effects of antineoplastic agents on axonal transport (2010)
  • Author(s): Goshima Y, Usui H, Shiozawa T, Hida T, Kuraoka S, Takeshita S, Yamashita N, Ichikawa Y, Kamiya Y, Gotoh T, Gotoh T.
  • Journal Title: Journal of Pharmacological Sciences Volume: 114(2) Pages: 168-79
  • DOI: DOI:10.1254/jphs.09352FP
• [Journal Article] Semaphorin3A signaling mediated by Fyn-dependent tyrosine phosphorylation of collapsin response mediator protein 2 at tyrosine 32 (2009)
  • Author(s): Uchida Y, Ohshima T, Yamashita N, Ogawara M, Sasaki Y, Nakamura F, Goshima Y.
  • Journal Title: J Biol Chem Volume: 284(40) Pages: 27393-401
  • DOI: DOI:10.1074/jbc.M109.000240
• [Journal Article] Increased proximal bifurcation of CA1 pyramidal apical dendrites in sema3A mutant mice (2009)
  • Author(s): Nakamura F, Ugajin K, Yamashita N, Okada T, Uchida Y, Taniguchi M, Ohshima T, Goshima Y.
  • Journal Title: J Comp Neurol Volume: 516(5) Pages: 360-75
  • DOI: DOI:10.1002/cne.22125
• [Presentation] Semaphorin3A drives AMPA receptor subunit GluR2 to dendrites by a retrograde transport of PlexinA4 along axon (2011)
  • Author(s): Yamashita N, Chen S, Goshima Y
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-09-15
• [Presentation] Semaphorin3A facilitates the axonal transport of PlexinA4 through interaction of PlexinA4 with TrkA (2011)
  • Author(s): Yamane M, Yamashita N, Sasaki Y, Nakamura F, Goshima Y
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-09-15
• [Presentation] Evidence for cross-talk between Sema3A and NGF signaling (2011)
  • Author(s): Yamane M, Yamashita N, Goshima Y
  • Organizer: 第84回日本薬理学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-03-23
• [Presentation] Semaphorin3A drives AMPA receptor subunit GluR2 to dendrites (2010)
  • Author(s): Yamashita N, Usui H, Chen S, Goshima Y
  • Organizer: Neuro2010
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2010-09-02
• [Presentation] Semaphorin3A drives AMPA receptor subunit GluR2 to dendrites (2009)
  • Author(s): Yamashita N, Usui H, Chen S, Goshima Y
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-09-16
• [Presentation] Comprehensive behavioral analysis of the crmp1 knockout mice (2009)
  • Author(s): Yamashita N, Takao K, Miyakawa T, Goshima Y
  • Organizer: Japan-United States Joint Seminar/2nd Cdk5 International Symposium
  • Place of Presentation: 首都大学東京
  • Year and Date: 2009-06-26
• [Book] 軸索ガイダンスおよびシナプス形成とCdk5.蛋白質・核酸・酵素 (2009)
  • Author(s): 山下直也, 中村史雄, 五嶋良郎
  • Total Pages: 802-807
  • Publisher: 共立出版
• [Remarks]
  • URL: http://www-user.yokohama-cu.ac.jp/～pharmac/