2010 Fiscal Year Final Research Report
Elucidation of the molecular mechanism for inhibition of let-7 miRNA function by RNA-binding protein Musashi1
| Project/Area Number |
21700413
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Keio University |
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Principal Investigator |
IMAI Takao Keio University, 医学部, 助教 (10383712)
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| Project Period (FY) |
2009 – 2010
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| Keywords | RNA結合蛋白質 / 神経幹細胞 / miRNA / 翻訳 |
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| Research Abstract |
Musashi1 is an RNA-binding protein, which is expressed in neural stem/progenitor cells. I revealed following new findings. (1) Musashi1 directly bound to let-7 micro RNAs with sequence-specific manner. (2)Musashi1 inhibited the translational repression of let-7 down-stream target gene thorough the let-7. (3) There was the molecular aspect that Ago2 and Musashi1 exist in the same protein complex. (4)Musashi1 promoted the protein degradation of PAZ domain. The inhibition of let-7 function by Musashi1 is possibly due to the Musashi1-induced PAZ degradation.
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