2010 Fiscal Year Final Research Report
Analysis of astrocytic function by using STOP-tetO system
| Project/Area Number |
21700419
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | National Institute for Physiological Sciences |
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Principal Investigator |
TANAKA Kenji National Institute for Physiological Sciences, 分子生理研究系, 助教 (30329700)
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| Project Period (FY) |
2009 – 2010
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| Keywords | グリア細胞 / アストロサイト / オリゴデンドロサイト / MLC |
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| Research Abstract |
I succeeded to establish the animal model for a white matter disease named megalencephalic leukoencephalopathy with subcortical cysts (MLC), which is caused by MLC1 mutation. I used a versatile new gene modulation system to generate both mouse Mlc1 gene conditional knockout and overexpression. I found that Mlc1 was exclusively expressed by astrocytes and its overexpression resulted in astrocytic swelling and vacuolating myelopathy, whereas Mlc1 gene knockout mice were grossly normal.
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