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2010 Fiscal Year Final Research Report

Analysis of CAPS2 gene function

Research Project

  • PDF
Project/Area Number 21700421
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Neurochemistry/Neuropharmacology
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

SADAKATA Tetsushi  The Institute of Physical and Chemical Research, 分子神経形成研究チーム, 研究員 (90391961)

Project Period (FY) 2009 – 2010
Keywords神経 / 分泌 / 自閉症
Research Abstract

CAPS protein regulates exocytosis of catecholamine- or neuropeptide-containing dense-core vesicles (DCVs) at secretion sites such as nerve terminals. However, large amounts of CAPS protein are localized in the cell soma, and the role of somal CAPS protein remains unclear. We showed that somal CAPS1 plays an important role in dense-core vesicle (DCV) trafficking in the trans-Golgi network. The anti-CAPS1 antibody appeared to pull down membrane fractions, including many Golgi-associated proteins such as ADP-ribosylation factor (ARF) small GTPases. Biochemical analyses of the protein-protein interaction showed that CAPS1 interacted specifically with the class II ARF4/ARF5, but not with other classes of ARFs, via the pleckstrin homology (PH) domain in a GDP-bound ARF form-specific manner. The PH domain of CAPS1 showed high affinity for the Golgi membrane, thereby recruiting ARF4/ARF5 to the Golgi complex. These findings implicate a functional role for CAPS1 protein in regulating DCV trafficking in the trans-Golgi network.

  • Research Products

    (13 results)

All 2010 2009

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (8 results)

  • [Journal Article] Calcium-dependent activator protein for secretion 2 (CAPS2) promotes BDNF secretion and is critical for the development of GABAergic interneuron network.2010

    • Author(s)
      Yo Shinoda, Tetsushi Sadakata, Kazuhito Nakao, Ritsuko Katoh-Semba, Emi Kinameri, Asako Furuya, Yuchio Yanagawa, Hajime Hirase, Teiichi Furuichi.
    • Journal Title

      Proceedings of the National Academy of Sciences. 108(1)

      Pages: 373-378

    • Peer Reviewed
  • [Journal Article] Interaction of CAPS1 with the class II ARF small GTPases is required for dense-core vesicle trafficking in the trans-Golgi network.2010

    • Author(s)
      Tetsushi Sadakata, Yo Shinoda, Yukiko Sekine, Chihiro Saruta, Makoto Itakura, Masami Takahashi, Teiichi Furuichi.
    • Journal Title

      Journal of Biological Chemistry. 285(49)

      Pages: 38710-38719

    • Peer Reviewed
  • [Journal Article] Ca2+-dependent activator protein for secretion 2 and autistic-like phenotypes2010

    • Author(s)
      Tetsushi Sadakata, Teiichi Furuichi.
    • Journal Title

      Neuroscience Research. 67(3)

      Pages: 197-202

    • Peer Reviewed
  • [Journal Article] Response to the letter by Eran et al. (Comment on "Autistic-like phenotypes in Cadps2-knockout mice and aberrant CADPS2 splicing in autistic patients")2009

    • Author(s)
      Tetsushi Sadakata, Teiichi Furuichi.
    • Journal Title

      Journal of Clinical Investigation. 119(4)

      Pages: 680-681

    • Peer Reviewed
  • [Journal Article] Developmentally-regulated Ca^<2+>-dependent activator protein for secretion 2 (CAPS2) is involved in BDNF secretion and is associated with autism susceptibility.2009

    • Author(s)
      Tetsushi Sadakata, Teiichi Furuichi.
    • Journal Title

      Cerebellum. 8(3)

      Pages: 312-322

    • Peer Reviewed
  • [Presentation] Autistic-like phenotypes in CAPS2 exon-skipped mice2010

    • Author(s)
      Tetsushi Sadakata
    • Organizer
      XVIIIth World Congress on Psychiatric Genetics
    • Place of Presentation
      Athens, Greece
    • Year and Date
      20101003-20101007
  • [Presentation] CAPS2のエキソン3スキップマウスは自閉症様形質を示す2010

    • Author(s)
      定方哲史
    • Organizer
      Neuro 2010
    • Place of Presentation
      神戸
    • Year and Date
      20100902-20100904
  • [Presentation] CAPS2による有芯小胞分泌と自閉症2010

    • Author(s)
      定方哲史
    • Organizer
      第27回東北大学脳科学グローバルCOE若手フォーラム
    • Place of Presentation
      仙台
    • Year and Date
      2010-08-27
  • [Presentation] CAPS2 protein-mediated regulation of dense-core vesicle secretion pathway and susceptibility to developmental disorders2010

    • Author(s)
      Tetsushi Sadakata
    • Organizer
      東北大学脳科学グローバルCOE・第3回脳神経科学サマーリトリート
    • Place of Presentation
      仙台
    • Year and Date
      2010-07-31
  • [Presentation] CAPS and ARFs are associated with dense-core vesicle biogenesis from the Golgi complex2009

    • Author(s)
      Tetsushi Sadakata
    • Organizer
      The Japan-Korea Conference on Cellular Signaling for Young Scientists
    • Place of Presentation
      長崎
    • Year and Date
      20091124-20091126
  • [Presentation] CAPS1とクラスII ARFはゴルジ体における有芯小胞の生成に関わる2009

    • Author(s)
      Tetsushi Sadakata
    • Organizer
      日本生化学会
    • Place of Presentation
      神戸
    • Year and Date
      20091021-20091024
  • [Presentation] The functional role of CAPS family proteins in dense-core vesicle trafficking2009

    • Author(s)
      Tetsushi Sadakata
    • Organizer
      日本神経科学学会
    • Place of Presentation
      名古屋
    • Year and Date
      20090916-20090918
  • [Presentation] ペプチドを含む有芯小胞はゴルジ体よりいかにして生まれる?2009

    • Author(s)
      Tetsushi Sadakata
    • Organizer
      日本神経内分泌学会
    • Place of Presentation
      北九州
    • Year and Date
      20090904-20090905

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Published: 2012-02-13   Modified: 2016-04-21  

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