2010 Fiscal Year Final Research Report

Basic study for production of efficient somatic cell cloned embryos

Research Project

Project/Area Number	21700455
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Laboratory animal science
Research Institution	National Institute of Biomedical Innovation
Principal Investigator	SHIMOZAWA Nobuhiro National Institute of Biomedical Innovation, 霊長類医科学研究センター, 研究員 (50300786)
Project Period (FY)	2009 – 2010
Keywords	クローン / 核移植 / 体外培養 / 単為発生 / 活性化
Research Abstract	Firstly, it was indicated that CMRL-1066 medium, generally using for macaque monkey embryos, were available as the culture medium of monkey-rabbit interspecies somatic cell nuclear transfer embryos. In addition, by nuclear transfer using cynomolgus monkey somatic cells arrested at metaphase of cell cycle by nocodazole, the metaphase stage was maintained in transferred rabbit oocyte. It was clarified that activation by a combination of ionomycin and cycloheximide was suitable for nuclear transfer using the somatic cells at metaphase. Development to 8-cell stage of the interspecies nuclear transfer embryos was improved by using MG132. In the animals that collection of many oocytes are difficult, these results showed that the interspecies nuclear transfer was available for examination of the basic techniques.