Analysis of trafficking mechanism of endothelial PECAM-1 to the spot of monocyte transmigration in atherogenesis.

2010 Fiscal Year Final Research Report

• Project/Area Number: 21700476
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Kawasaki Medical School
• Principal Investigator: HASHIMOTO Ken Kawasaki Medical School, 医学部, 助教 (80341080)
• Project Period (FY): 2009 – 2010
• Keywords: 動脈硬化 / 血管内皮細胞 / 単球 / 浸潤 / PECAM-1 / VE-cadherin

Research Abstract

In atherogenesis, it is unclear whether and how one monocyte transmigration event affects endothelium to facilitate subsequent ones. When human monocytes were added twice to endothelial cells in vitro, significant augmentation of transmigration was observed at the second addition. Endothelial surface expressions of two major junctional molecules, PECAM-1 and VE-cadherin, increased and decreased respectively, in response to monocyte addition. These findings show that monocyte trans-endothelial migration alters endothelial junctional organization to more monocyte-permeable state (increased PECAM-1 and decreased VE-cadherin), resulting in the augmented transmigratory activity at a later stage.

Research Products

• [Journal Article] Monocyte trans-endothelial migration augments subsequent transmigratory activity with increased PECAM-1 and decreased VE-cadherin at endothelial junctions. (2010)
  • Author(s): Hashimoto K, Kataoka N, Nakamura E, Hagihara K, Hatano M, Okamoto T, Kanouchi H, Minatogawa Y, Mohri S, Tsujioka K, Kajiya F.
  • Journal Title: Int J Cardiol. in press
  • Peer Reviewed
• [Journal Article] An Experimental Model for Studying Molecular Behavior of Platelet-Endothelial Cell Adhesion Molecule-1 during Mechanical Interactions between Monocytes and Vascular Endothelial Cells (2010)
  • Author(s): Ken HASHIMOTO, Noriyuki KATAOKA, Emi NAKAMURA, Takeaki OKAMOTO, Hiroaki KANOUCHI, Yohsuke MINATOGAWA, Satoshi MOHRI, Katsuhiko TSUJIOKA, Fumihiko KAJIYA
  • Journal Title: Journal of Biomechanical Science and Engineering 5(4) Pages: 281-290
  • Peer Reviewed
• [Journal Article] Altered nano/micro-order elasticity of pulmonary artery smooth muscle cells of patients with idiopathic pulmonary arterial hypertension. (2010)
  • Author(s): Nakamura K, Shimizu J, Kataoka N, Hashimoto K, Ikeda T, Fujio H, Ohta-Ogo K, Ogawa A, Miura A, Mohri S, Nagase S, Morita H, Kusano KF, Date H, Matsubara H, Mochizuki S, Hashimoto K, Kajiya F, Ohe T.
  • Journal Title: Int J Cardiol. 140(1) Pages: 102-107
  • Peer Reviewed
• [Journal Article] Evaluation of Coronary Endothelial Function by Catheter-Type NO Sensor in High-Fat-Diet-Induced Obese Dogs. (2009)
  • Author(s): Kume T, Kawamoto T, Okura H, Neishi Y, Hashimoto K, Hayashida A, Watanabe N, Kanda Y, Mochizuki S, Goto M, Yoshida K.
  • Journal Title: Circ J. 73(3) Pages: 562-567
  • Peer Reviewed
• [Journal Article] Measurement and finite element modeling of the force balance in the vertical section of adhering vascular endothelial cells. (2009)
  • Author(s): Shinji Deguchi, Hiroyuki Fukamachi, Ken Hashimoto, Kazushi Iio, Katsuhiko Tsujioka.
  • Journal Title: Journal of the Mechanical Behavior of Biomedical Materials 2 Pages: 173-185
  • Peer Reviewed
• [Presentation] 単球の内皮下浸潤により誘発される内皮細胞間隙分子の変化が次の単球浸潤を促進する (2010)
  • Author(s): 橋本謙, 片岡則之, 辻岡克彦, 梶谷文彦, 毛利聡
  • Organizer: 第62回日本生理学会中国四国地方会
  • Place of Presentation: 出雲
  • Year and Date: 20101120-20101121
• [Presentation] Core 5.Myocardium : Function and Failure Session Title : Ventricular Function, Mechanics and Genes Abstract 13933 : Increased Cardiomyocyte Stiffness in the Transverse Direction and Incomplete Relaxation in Hypertrophied Rat Hearts Induced by Chronic β-adrenergic Stimulation (2010)
  • Author(s): Kazufumi Nakamura, Wakako S Yoshikawa, Daiji Miura, Juichiro Shimizu, Ken Hashimoto, Noriyuki Kataoka, Hiroko Toyota, Hiroshi Okuyama, Norihisa Toh, Hiroshi Morita, Kengo F Kusano, Tatsuhito Matsuo, Miyako Takaki, Naoto Yagi, Hiroshi Ito.
  • Organizer: American Heart Association's Scientific Sessions 2010
  • Place of Presentation: Chicago, USA
  • Year and Date: 20101113-20101117
• [Presentation] 単球の内皮下浸潤によって誘発される次の単球浸潤の促進 (2010)
  • Author(s): 橋本謙, 片岡則之, 毛利聡, 辻岡克彦, 梶谷文彦
  • Organizer: 第25回生体・生理工学シンポジウム, The 25th Symposium on Biological and Physiological Engineering(オーガナイズドセッション「生体機能計測の新展開」,論文集, 469-470)
  • Place of Presentation: 岡山
  • Year and Date: 20100923-20100925
• [Presentation] 動脈硬化における単球及び血管内皮細胞のダイナミクス-培養細胞を用いたバイオイメージング-(In vitro bioimaging of monocyte trans-endothelial migration in early atherogenesis)バイオイメージング,Vol.19(2) (2010)
  • Author(s): 橋本謙, 片岡則之, 毛利 聡, 辻岡克彦, 梶谷文彦
  • Organizer: 第19回日本バイオイメージング学会学術集会要旨集
  • Place of Presentation: 横浜,シンポジウム
  • Year and Date: 20100909-20100911
• [Presentation] Monocyte trans-endothelial migration induces PECAM-1 accumulation and VE-cadherin reduction at the surface of vascular endothelial cells. (2010)
  • Author(s): Ken Hashimoto, Noriyuki Kataoka, Emi Nakamura, Kimiko Hagihara, Takeaki Okamoto, Hiroaki Kanouchi, Yohsuke Minatogawa, Satoshi Mohri, Katsuhiko Tsujioka, Fumihiko Kajiya.
  • Organizer: 6th World Congress on Biomechanics, Singapore Suntec Convention Centre 148
  • Place of Presentation: abstract
  • Year and Date: 20100801-20100806
• [Presentation] Experimental Model for Studying Molecular Behavior in Endothelial Cells during Interaction with Monocytes. (2010)
  • Author(s): 片岡則之, 橋本謙, 毛利聡, 梶谷文彦
  • Organizer: 第49回日本生体医工学会大会(細胞バイオメカニクスの新しい展開」,プログラム・抄録集 219)
  • Place of Presentation: 大阪,シンポジウム(CD-ROM S-10-4)
  • Year and Date: 20100625-20100627
• [Presentation] Increased Cardiomyocyte Stiffness in Transverse Direction in Hypertrophied Rat Hearts Induced by Chronic B-adrenergic Stimulation. Circulation Journal 74 Suppl.I, 263-264, 2010 (2010)
  • Author(s): Kazufumi Nakamura, Daiji Miura, Ken Hashimoto, Hiroko Toyota, Hiroshi Okuyama, Juichiro Shimizu, Wakako Sumida, Satoshi Nagase, Kunihisa Kohno, Hiroshi Morita, Kengo Kusano, Naoto Yagi, Hiroshi Ito
  • Organizer: 第74回日本循環器学会
  • Place of Presentation: 京都
  • Year and Date: 20100305-20100307
• [Presentation] 肺動脈平滑筋細胞と心筋細胞におけるnano/microオーダーの弾性の変化-原子間力顕微鏡による検討- (2010)
  • Author(s): 中村一文, 三浦大志, 片岡則之, 橋本謙, 草野研吾, 伊藤浩
  • Organizer: 第22回バイオエンジニアリング講演会講演論文集 374
  • Place of Presentation: 岡山
  • Year and Date: 20100109-20100110
• [Presentation] 細胞間隙を介する単球の浸潤により誘発される内皮細胞PECAM-1の浸潤部位への持続的集積 (2010)
  • Author(s): 橋本謙, 片岡則之, 毛利聡, 梶谷文彦
  • Organizer: 第22回バイオエンジニアリング講演会講演論文集 216
  • Place of Presentation: 岡山
  • Year and Date: 20100109-20100110
• [Presentation] Increased Cardiomyocyte Elasticity in the Transverse Direction in Hypertrophied Rat Hearts Induced by Chronic s-Adrenergic Stimulation. (2009)
  • Author(s): Kazufumi Nakamura, Daiji Miura, Wakako S Yoshikawa, Ken Hashimoto, Noriyuki Kataoka, Juichiro Shiizu, Nobuhiro Nishii, Satoshi Nagase, Hiroshi Morita, Yoshiki Hata, Kengo F Kusano, Hiroshi Ito.
  • Organizer: Circulation, 2009; 120 : S1147. American Heart Association's Scientific Sessions 2009
  • Place of Presentation: Orlando, Florida, USA
  • Year and Date: 20091214-20091218
• [Presentation] Role of dynamic recruitment of Endothelial PECAM-1 to Transmigrating Monocytes.(浸潤する単球への内皮細胞PECAM-1の局所集積),生物物理,Vol 49, Suppl.1, S-57, 2009 (2009)
  • Author(s): Noriyuki Kataoka, Ken Hashimoto, Satoshi Mohri, Fumihiko Kajiya.
  • Organizer: 第47回日本生物物理学会
  • Place of Presentation: 徳島
  • Year and Date: 20091030-20091101
• [Presentation] ENDOTHELIAL TRANSIENT RECEPTOR POTENTIAL VANILLOID CHANNEL 1 AND SMALL CONDUCTANCE POTASSIUM CHANNEL STRENGTHENED ADHESION OF MONOCYTE.J.Physiol.Sci.59 (Suppl.1), p500, 2009 36th Congress of the International Union of Physiological Sciences (IUPS2009) (2009)
  • Author(s): Naoyuki Himi, Airi Hamaguchi, Ken Hashimoto, Tomoshige Koga, Katsuhiko Tsujioka.
  • Organizer: 第86回日本生理学会大会
  • Place of Presentation: 京都
  • Year and Date: 20090727-20090801
• [Presentation] MONOCYTE INVASION INDUCES REDISTRIBUTION AND SUBSEQUENT ACCUMULATION OF ENDOTHELIAL PECAM-1 DURING PARA-, BUT NOT TRANS-CELLULAR MIGRATION. J.Physiol.Sci.59 (Suppl.1), p324, 2009 36th Congress of the International Union of Physiological Sciences (IUPS2009) (2009)
  • Author(s): Ken Hashimoto, Noriyuki Kataoka, Emi Nakamura, Kimiko Hagihara, Takeaki Okamoto, Hiroaki Kanouchi, Yohsuke Minatogawa, Satoshi Mohri, Katsuhiko Tsujioka, Fumihiko Kajiya.
  • Organizer: 第86回日本生理学会大会
  • Place of Presentation: 京都
  • Year and Date: 20090727-20090801
• [Presentation] 細胞間隙を通る単球の浸潤により誘発される内皮細胞PECAM-1の再配置及び浸潤後の持続的集積生体医工学 Vol.47, Suppl.1, p331, 2009. (2009)
  • Author(s): 橋本謙, 片岡則之, 中村恵美, 萩原喜美子, 毛利聡, 辻岡克彦, 梶谷文彦
  • Organizer: 第48回日本生体医工学会大会
  • Place of Presentation: 東京,CD-ROM
  • Year and Date: 20090423-20090425