2010 Fiscal Year Final Research Report
Study on the mechanism of the differentiation control of osteoblast and osteoclast based on the surface characteristics of biomaterials for hard tissues
| Project/Area Number |
21700485
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
NAKAMURA Miho Tokyo Medical and Dental University, 生体材料工学研究所, 助教 (40401385)
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| Project Period (FY) |
2009 – 2010
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| Keywords | バイオマテリアル / 骨芽細胞 / 破骨細胞 |
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| Research Abstract |
The purpose of this study was to study the surface characteristics of polarized hydroxyapatite (HA) and the effects of electrical stimulation by polarization on osteoblast behavior in vitro in order to investigate the mechanism of the osteoconductive enhancement. Based on the material analysis, the surface characteristics revealed that electrical polarization had no effect on surface roughness, crystallinity, and constituent elements. According to contact angle measurement, electrically polarized HA, which provides two kinds of surfaces, negatively charged HA (N-HA) and positively charged HA (P-HA), was even more hydrophilic than that of normal HA. Morphological observations and quantitative analyses revealed that the typical adhered cells had a round shape on the HA but had a spindle or fan-like spreading configuration on the N-HA and the P-HA. The cell areas positively stained for actin, which indicates the degree of cell spreading, were distinctly larger on the N-HA and P-HA than that on the HA. In conclusion, electrical polarization improved the wettability of the HA surface and affected osteoblastic adhesion, especially spreading.
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