2010 Fiscal Year Final Research Report
Effective preservation of stem cells with novel developed cryoprotectant
| Project/Area Number |
21700488
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kyoto University |
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Principal Investigator |
MATSUMURA Kazuaki Kyoto University, 再生医科学研究所, 研究員 (00432328)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 生体機能材料 / 凍結保存 |
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| Research Abstract |
We synthesized carboxylated poly-L-lysine by the reaction of poly-L-lysine (PLL) with succinic anhydride for the cryoprotective agents of living cells. Culture medium solutions of PLL with 65 mol% of amino groups carboxylated showed excellent post-thaw survival efficiency of various cell lines and rat, mouse, human mesenchymal stem cells and mouse and human induced pluripotent stem cells. Thus those polyampholytes can replace DMSO as new materials for cryoprotective agents. We elucidated the mechanisms of cryoprotective properties of those polyampholytes. The mechanisms would be involved with the membrane protective activity and inhibition of rapid increase of osmolarity during freezing by ion trapping to those polyampholytes.
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