2010 Fiscal Year Final Research Report
Creation of thermoresponsive nano-phase separated surfaces for cell adhesion and detachment
| Project/Area Number |
21700493
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
NAKAYAMA Masamichi Tokyo Women's Medical University, 医学部, 講師 (00338980)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 温度応答性高分子 / ブロックコポリマー / RAFT重合 / 細胞コーティング / 組織工学 / ポリ(N-イソプロピルアクリルアミド) |
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| Research Abstract |
This study focused on the fabrication of thermoresponsive cell culture surfaces through the deposition of thermoresponsive block copolymers on solid surfaces for thermally regulated cell adhesion and detachment. Grafted polymer amounts, thickness, and the chain length of thermoresponsive polymers significant affected the temperature-controlled cell adhesion/detachment. By optimizing these factors, confluently cultured cells were successfully harvested as sheet-like cellular architectures by reducing temperatures.
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