Relation of exercise training, blood sugar level and muscle capillaries network with type 2 diabetes mellitus

2010 Fiscal Year Final Research Report

• Project/Area Number: 21700559
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Rehabilitation science/Welfare engineering
• Research Institution: Himeji Dokkyo University
• Principal Investigator: MURAKAMI Shinichiro Himeji Dokkyo University, 医療保健学部, 講師 (30454763)
• Project Period (FY): 2009 – 2010
• Keywords: 糖尿病 / 運動療法 / 血糖値 / 微小循環

Research Abstract

The aerobic exercise training has long been utilized as one of therapy in the type 2 diabetes mellitus. The effects of a therapeutic exercise on the myocyte and the microcirculation of skeletal muscle in diabetic rats were investigated. The aerobic exercise program consisted of 2 weeks running on a treadmill (0°incline, 10m/min, 60min/day, 6day/wk). Treated diabetic rats exhibited a decrease in plasma glucose, glycohemoglobin, and reactive oxygen species (ROS) compared with those of the diabetic rats but did not completely normalize levels. Usually, in the soleus muscles of the non-diabetic rats were composed by higher percentage of type I MyHC isoform and lower percentage of type II MyHC isoform. But there were no type II fibers in the soleus muscles of the diabetic rats. The succinate dehydrogenase (SDH) activity in training diabetic rat was significantly higher than that in diabetic rat. The capillary volume in diabetic muscle was significantly less. In the aerobic exercised diabetic muscles, the capillary volumes were increased toward control levels and completely normalize levels. In summary, the results of the present study provide evidence for the effectiveness of exercise training in reducing some of the muscle circulatory complications associated with diabetes mellitus.

Research Products

• [Journal Article] The Combined Effect of Electrical Stimulation and Resistance Isometric Contraction on Muscle Atrophy in Rat Tibialis Anterior Muscle. (2011)
  • Author(s): Fujita N, Murakami S, Arakawa T, Miki A, Fujino H.
  • Journal Title: Bosn J Basic Med Sci. 11(2) Pages: 1-6
  • Peer Reviewed
• [Journal Article] Comparision of Capillary Architecture between Slow and Fast Muscles in Rats Using a Cofocal Laser Scanning Microscope. (2010)
  • Author(s): Murakami S, Fujino H, Takeda I, Momota R, Kumagishi K, Ohtsuka A.
  • Journal Title: Acta Medica Okayama. 64(1) Pages: 11-18
  • Peer Reviewed
• [Journal Article] 後肢懸垂前の温熱療法が筋に及ぼす影響 (2009)
  • Author(s): 田尻直輝, 武田功, 藤野英己, 村上慎一郎, 松永秀俊, 小幡太志, 高見博文, 森彩子, 森経介, 小原教考
  • Journal Title: 理学療法岡山 No,29 Pages: 4-6
• [Journal Article] 運動習慣と運動中止が体重増加に与える影響 (2009)
  • Author(s): 森彩子, 武田功, 藤野英己, 村上慎一郎, 小幡太志, 高見博文, 田尻直輝, 松永秀俊, 石井禎基, 小田桐匡, 森経介, 小原教考
  • Journal Title: 理学療法岡山 No,29 Pages: 12-15
• [Journal Article] Thermal Preconditioning Prevents Fiber Type Transformation of The Unloading Induced-atrophied Muscle in Rats. (2009)
  • Author(s): Takeda I, Fujino H, Murakami S, Kondo H, Nagatomo F, Ishihara A.
  • Journal Title: J Muscle Res Cell Motil. 30(3-4) Pages: 145-152
  • Peer Reviewed
• [Presentation] Abnormality of Myosin Heavy Chain Isoform Type and Succinatedehydrogenase Activity in Soleus Muscle of Diabetic Rat. (2011)
  • Author(s): Murakami S, Kondo H, Fujita N, FujinoH.
  • Organizer: 16th International WCPT Congress
  • Place of Presentation: Amsterdam
  • Year and Date: 20110620-20110623
• [Presentation] Therapeutic Exercise Attenuates Microangiopathy of Skeletal Muscle in type II Diabetes. (2011)
  • Author(s): Fujino H, Murakami S, Kondo H, Fujita N, Nagatomo F, Takeda I, Ishihara A
  • Organizer: 16th international WCPT Congress
  • Place of Presentation: Amsterdam
  • Year and Date: 20110620-20110623
• [Presentation] Effect on Capillary of Aerobics Exercise to Diabetic Muscle. (2011)
  • Author(s): Murakami S, Kondo H, Fujita N, Fujino H.
  • Organizer: ACSM 58th Annual Meeting and 2nd World Congress on Exercise is Medicine
  • Place of Presentation: Denver
  • Year and Date: 20110501-20110604
• [Presentation] High-intensity Exercise Training Attenuates Diabetes-induced Muscle Capillary Regression in Non-obese NIDDM Rats. (2011)
  • Author(s): Fujino H, Kondo H, Murakami S, Fujita N, Nagataomo F, Takeda I, Ishihara A, Roland R.Roy.
  • Organizer: ACSM 58th Annual Meeting and 2nd World Congress on Exercise is Medicine
  • Place of Presentation: Denver
  • Year and Date: 20110501-20110604
• [Presentation] Low-intensity Exercise Prevents Hyperglycemia and Hyperlipidemia in Spontaneously Type 2 Diabetic Torii rat. (2011)
  • Author(s): Kondo H, Fujino H, Murakami S, Fujita N, Morifuji T, Shiina S, Nagatomo F, Ishihara A.
  • Organizer: ACSM 58th Annual Meeting and 2nd World Congress on Exercise is Medicine
  • Place of Presentation: Denver
  • Year and Date: 20110501-20110604
• [Presentation] Low-Intensity Exercise Training Prevents Muscular Microangiopathy in Non-obese Type 2 Diabetic Rats. (2011)
  • Author(s): Fujino H, Kondo H, Murakami S, Fujita N, Morifuji T, Nagatomo F, Ishihara A.
  • Organizer: Experimental biology 2011
  • Place of Presentation: Washington D.C.
  • Year and Date: 20110409-20110413
• [Presentation] Effects of Exercise Preconditioning on Capillary in Soleus Muscle of Type 2 Diabetic Rats. (2011)
  • Author(s): Murakami S, Morifuji T, Kondo H, Takeda I, Fujita N, FujinoH.
  • Organizer: Experimental biology 2011
  • Place of Presentation: Washington D.C.
  • Year and Date: 20110409-20110413
• [Presentation] Decreased Capillary Volume and Angiogenic Factors in Type 2 Diabetic Skeletal Muscle. (2010)
  • Author(s): Kondo H, Fujino H, Murakami S, Nagatomo F, Ishihara A.
  • Organizer: The 50th annual meeting of American society for cell biology
  • Place of Presentation: Philadelphia
  • Year and Date: 20101111-20101115
• [Presentation] Threedimensional visualization of capillary and exercise-induced expression of angiogenic growth factors in skeletal muscle of non-insulin-dependent diabetes. (2010)
  • Author(s): Fujino H, Kondo H, Murakami S, Fujita N, Nagatomo F, Ishihara A.
  • Organizer: The 50 th annual meeting of American society for cell biology
  • Place of Presentation: Philadelphia
  • Year and Date: 20101111-20101115
• [Presentation] Capillarity and angiogenic factors in soleus muscle associated with type II diabetes mellitus. (2010)
  • Author(s): Kondo H, Fujino H, Murakami S, Nagatomo F, Ishihara A.
  • Organizer: ACSM conference on Integrative physiology of exercise 2010
  • Place of Presentation: Miami
  • Year and Date: 20100922-20100925
• [Presentation] Endurance exercise attenuates microangiopathy of muscle capillary in non-insulin-dependent diabetic rat. (2010)
  • Author(s): Fujino H, Kondo H, Murakami S, Fujita N, Nagatomo F, Takeda I, Ishihara A.
  • Organizer: ACSM conference on Integrative physiology of exercise 2010.
  • Place of Presentation: Miami
  • Year and Date: 20100922-20100925
• [Presentation] The influence of angiogenic and anti-angiogenic factors on the degradation of the capillary in Type II diabetic rat. (2010)
  • Author(s): Kondo H, Fujino H, Murakami S, Nagatomo F, Ishihara A.
  • Organizer: 第18回日本運動生理学会大会
  • Place of Presentation: 鹿児島
  • Year and Date: 20100731-20100801
• [Presentation] Effects of endurance exercise on muscle capillary network in non-insulin-dependent diabetic rats. (2010)
  • Author(s): Fujino H, Murakami S, Kondo H, Fujita N, Nagatomo F, Ishihara A.
  • Organizer: 第18回日本運動生理学会大会
  • Place of Presentation: 鹿児島
  • Year and Date: 20100731-20100801
• [Presentation] Exercise preconditioning attenuates oxidative stress and hyperglycemia in spontaneously diabetic torii rat. (2010)
  • Author(s): Kondo H, Fujino H, Murakami S, Nagatomo F, Takeda I, Ishihara A.
  • Organizer: 57 th Annual meeting of American college of sports medicine
  • Place of Presentation: Boltimore
  • Year and Date: 20100601-20100605
• [Presentation] Capillary hemodynamics and expression of angiogenic regulators in metabolically different rat muscles. (2010)
  • Author(s): Fujino H, Kondo H, Murakami S, Nagatomo F, Takeda I, Ishihara A, Roy RR.
  • Organizer: 57th American college of sports medicine
  • Place of Presentation: Baltimore
  • Year and Date: 20100601-20100605
• [Presentation] 非インスリン依存型糖尿病モデルラットのヒラメ筋ミオシン重鎖アイソフォームのタイプ移行と酸化的リン酸化活性の変化 (2010)
  • Author(s): 村上慎一郎, 藤野英己, 近藤浩代, 小幡太志, 弓影秀樹, 高見博文, 松永秀俊, 武田功
  • Organizer: 第45回日本理学療法学術大会
  • Place of Presentation: 岐阜
  • Year and Date: 20100527-20100529
• [Presentation] Fiber capillary regression and endothelial apoptosis against disuse atrophy in rat soleus muscle : role of angiogenic regulators. (2010)
  • Author(s): Fujino H, Kondo H, Murakami S, Nagatomo F, Takeda I, Ishihara A, Roy RR.
  • Organizer: Experimental Biology 2010
  • Place of Presentation: Anaheim
  • Year and Date: 20100424-20100428
• [Presentation] Abnormality of capillarity and oxidative enzyme activity in slow muscle of type 2 diabetic rats. (2010)
  • Author(s): Murakami S, Fujino H, Kondo H, Takeda I, Momota R, Kumagishi K, Ohtsuka A.
  • Organizer: Experimental Biology 2010
  • Place of Presentation: Anaheim
  • Year and Date: 20100424-20100428
• [Presentation] Responses of pro-and anti-angiogenic factors to capillary in rat soleus muscle after hindlimb unloading. (2009)
  • Author(s): Fujino H, Kondo H, Murakami S, Nagatomo F, Takeda I, Ishihara A.
  • Organizer: 49th The American Society for Cell Biology
  • Place of Presentation: San Diego
  • Year and Date: 20091200
• [Presentation] Edgerton VR : Implications of angiogenic factors and thrombospondin-1 underlying capillary regression in chronically unloaded rat soleus muscle. (2009)
  • Author(s): Fujino H, Ishihara A, Murakami S, Kondo H, Zhong H, Roy RR
  • Organizer: American college of sports medicine
  • Place of Presentation: Seattle
  • Year and Date: 20090500