The effect of L-ascorbic acid on transcriptional regulation

2011 Fiscal Year Final Research Report

• Project/Area Number: 21700751
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Eating habits, studies on eating habits
• Research Institution: Ochanomizu University
• Principal Investigator: SONE Yasuko お茶の水女子大学, 生活環境教育研究センター, 助教 (80452027)
• Project Period (FY): 2009 – 2011
• Keywords: L-アスコルビン酸 / ナトリウム依存性ビタミンCタランスポーター / デヒドロア / ChIP

Research Abstract

We investigated the effects of L-ascorbic acid(AsA) administration(0, 5 and 100 mg/day) on the expression levels of sodium-dependent vitamin C transporters(SVCTs) and that of other genes in ODS rat livers. Similarly, the effect of AsA addition into the medium on the transporters expressions in rat primary hepatocyte and HepG2 was examined. Moreover, to examine the interaction between the promoter regions of SVCTs and the transcriptional factors, ChiP IP & qPCR assay was conducted.
As a result, the SVCT1 levels increased in the livers of rats not administered AsA and decreased in the livers of rats administered 100 mg/day AsA, indicating that these transporter levels can be influenced by the amount of AsA administered. Also, these transporter levels can be associated with redox in vivo. The ChiP IP & qPCR assay exhibited the binding between of C/EBP and the promoter region of SVCT2.

Research Products

• [Journal Article] The level of orally ingested vitamin Caffected the expression of vitamin Ctransporters and vitamin Caccumulation in the livers of ODS rats (2011)
  • Author(s): Sone Y, Ueta E, Kodama S, Sannoumaru Y, Miyake N, Sone H, Fujiwara Y, Otsuka Y, Kondo K, Inagaki M, Namba E, Kurata T, Suzuki E
  • Journal Title: Biosci Biotechnol Biochem Volume: 75 Pages: 2394-7
  • Peer Reviewed
• [Journal Article] Dose of 3-methylcholanthrene enhancesvitamin C accumulation and mRNA expression of its transporter in theliver of ODS rats and in HepG2 cells (2011)
  • Author(s): Sone Y, Ueta E, Sannomaru Y, Miyake N, Sone H, Otsuka Y, Kondo K, Kurata T, Suzuki E
  • Journal Title: J Biochem Mol Toxicol Volume: 25 Pages: 369-76
  • Peer Reviewed
• [Presentation] Effect of 3-MC and L-Ascorbic Acid onGene Expression of Primary Human Hepatocyte (2009)
  • Author(s): Sone Y, Iwaki E, Otsuka Y
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 20091200
• [Presentation] Influence of Dose Level of Vitamin C onthe Expression of Vitamin C Transporters in ODS Rats (2009)
  • Author(s): Sone Y, Midorikawa A, Taniguchi S, Watanabe M, Shu M, Onitake F, Kodama S, Saito K, Sannoumaru Y, Ueta E, Miyake N, Sone H, Namba E, Otsuka Y, Kondo K, Inagaki M, Kurata T, Suzuki E
  • Organizer: 19^<th> InternationalCongress of Nutrition
  • Place of Presentation: Bangkok, Thailand
  • Year and Date: 20091000
• [Presentation] アスコルビン酸濃度の違いによる遺伝子発現変化の検討 (2009)
  • Author(s): 曽根保子、丑田晴菜、児玉暁、齋藤和美、山王丸靖子、三宅紀子、曽根博仁、倉田忠男、鈴木恵美子
  • Organizer: 日本ビタミン学会第61回大会
  • Place of Presentation: 京都
  • Year and Date: 20090500
• [Remarks]
  • URL: http://www.hles.ocha.ac.jp/food/eiyoken/index.html