2012 Fiscal Year Final Research Report
Clinical application of flavonoids as a support for pharmacotherapy
| Project/Area Number |
21700772
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Eating habits, studies on eating habits
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| Research Institution | Osaka Ohtani University |
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Principal Investigator |
MIURA Takeshi 大阪大谷大学, 薬学部, 助教 (60434809)
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| Project Period (FY) |
2009 – 2012
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| Keywords | 特殊栄養 / フラボノイド / 薬物治療 / 相互作用 |
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| Research Abstract |
Bacterial expression and purification of CBR1, CBR3 and AKR1A1 fused with 6xHis cluster was successfully carried out. Kinetic analyses revealed that some extracts from fruit or vegetable strongly inhibit the enzymatic activities of CBR1 with IC50 of approximately 0.03% (v/v). Furthermore, the inhibitory effects were detected in cells. One of food additives, BHA, was found to enhance the transcriptional activity of CBR1 gene through the activation of transcription factor, Nrf2. Isatin, a substrate of CBR1, was found to exacerbate kidney ischemia/reperfusion injury in rats, suggesting that CBR1 is a novel potential target for pharmacotherapy.
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