2011 Fiscal Year Final Research Report
Demonstration of persistent activation of ATM on translocated chromosomes by Foci on FISH method.
| Project/Area Number |
21710061
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Nagasaki University |
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Principal Investigator |
YAMAUCHI Motohiro 長崎大学, 大学院・医歯薬学総合研究科, 助教 (60437910)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 応答 / ATM |
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| Research Abstract |
I established" Foci on FISH" method, which combines immunofluorescence staining of phosphorylated histone H2AX and fluorescence in situ hybridization(FISH) of chromosomes. Using this technique, I demonstrated that H2AX phosphorylation, which indicates ATM activation, persists on dicentric chromosomes, a kind of chromosome translocation. Moreover, the present study revealed that ATM suppresses frequency of ionizing radiation-induced chromosome translocation via p53-dependent cell cycle checkpoint and DNA-PK-dependent DNA double-strand break repair pathway.
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Research Products
(10 results)
