2011 Fiscal Year Final Research Report
Moleculae basis of al kyl phosphate triester degradation by microorganisms
| Project/Area Number |
21710075
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Environmental technology/Environmental materials
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| Research Institution | Nagaoka University of Technology |
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Principal Investigator |
ABE Katsumasa 長岡技術科学大学, 工学部, 助教 (40509551)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 環境修復技術 / 環境汚染物質 / 有機リン化合物 / 加水分解酵素 / 発現機構 / 遺伝子クローニング |
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| Research Abstract |
Tris(1, 3-dichloro-2-propyl) phosphate(TDCPP), a typical organophosphate triesters, has been used worldwide as flame-retardants, and thus resulting in its dispersion into natural environment. Since this compound is known to possess carcinogenicity, mutagenicity and neurotoxicity, persistence of TDCPP in natural environment has been concerned. In this study, I report the purification and characterization of TDCPP-degrading enzyme from Sphingomonas sp. TDK1 and its gene cloning and sequencing. The enzyme can degrade not only the trihaloalkyl phosphates but also aryl organophosphates. The enzyme was induced when environmental orthophosphate becomes limiting.
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[Journal Article] Ryo-hei Yamada and Yoshio Kera : Isolation and identification of persistent chlorinated organophosphorus flame retardants-degrading bacteria2010
Author(s)
Shouji Takahashi, Ikuko Satake, Isao Konuma, Koji Kawashima, Manami Kawasaki, Shingo Mori, Jun Morino, Junich Mori, Hongde Xu, Katumasa Abe, Ryo-hei Yamada and Yoshio Kera
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Journal Title
Applied and Environmental Microbiology
Volume: 76
Pages: 5292-5296
DOI
Peer Reviewed
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