2010 Fiscal Year Final Research Report

Conformational dynamics measurement of EGFR by high-speed single-molecule FRET measurement

Research Project

Project/Area Number	21710122
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Nanomaterials/Nanobioscience
Research Institution	The Institute of Physical and Chemical Research
Principal Investigator	OKAMOTO Kenji The Institute of Physical and Chemical Research, 佐甲細胞情報研究室, 研究員 (40402763)
Research Collaborator	HIBINO Kayo 独立行政法人理化学研究所, 佐甲細胞情報研究室, 研究員 (40435673)
Project Period (FY)	2009 – 2010
Keywords	EGFR / 単一分子計測 / FRET / 統計的データ解析
Research Abstract	Single molecule FRET measurement on conformational dynamics of the EGFR molecule, which is related to signal transduction on the cell membrane, has been developed. Conditions for preparation and fluorescence labeling of EGFR were optimized and FRET signals were successfully observed while the dynamics measurements could have not been completed since it had been taken longer time to optimize EGFR expression conditions than initial expectation. A new method of experimental data analysis based on the variational Bayes was also developed, which improved the accuracy and the time resolution.

(2 results)