2011 Fiscal Year Final Research Report
Microenvironment array chip for cell culture environment screening
| Project/Area Number |
21710133
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Microdevices/Nanodevices
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
HATTORI Koji 独立行政法人産業技術総合研究所, 幹細胞工学研究センター, 産総研特別研究員 (60409670)
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| Project Period (FY) |
2009 – 2011
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| Keywords | マイクロバイオシステム / 薬剤開発 / 細胞アッセイ / 培養環境 / Lab on a chip / タンパク質表面修飾 / マイクロパターニング |
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| Research Abstract |
Generally, cellular events(e. g., adhesion, extension, growth, apoptosis and differentiation) strongly depend on extracellular stimuli from surrounding environment composed of soluble factors and scaffolds(i. e., extracellular matrix(ECM)). In this research, we developed the "microenvironment array chip" as a new-type of the perfusion culture microchamber array chip, where cells are cultured in the discrete microchambers with the different environment composed of combination of ECMs and soluble factors. We present the high-throughput cell culture condition screening by the microenvironment array. In addition, modification of cell culture surfaces on microfluidic devices is a big issue in the field of MicroTAS. However, the micropatterning process of ECM spots on the bottom of the discrete microchambers is complicated. We developed a simple and scalable fabrication process for micropatterning of ECM in the microchamber array by O_2 plasma oxidation with physical masking. The fabrication process includes coating, O_2 plasma oxidation with physical masking, and subsequent sealing. All these process are enough simple to enable scalable production of microchamber array with ECMs.
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