Functional analyses of antisense transcripts in the epigenetic regulation of gene expression

2010 Fiscal Year Final Research Report

• Project/Area Number: 21710196
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: 基礎ゲノム科学
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: SHIBA Hiroshi Nara Institute of Science and Technology, バイオサイエンス研究科, 助教 (20294283)
• Project Period (FY): 2009 – 2010
• Keywords: DNAメチル化 / アンチセンス転写産物 / メチローム / ゲノムタイリングアレイ

Research Abstract

DNA methylation within the gene body is thought to impede transcriptional elongation, but the causal role of genic DNA methylation remains essentially unknown. In this research, I have generated DNA methylation profile (methylome) as well as sense and antisense transcription profiles (transcriptome), at 35-base pair resolution, of the wild-type and the loss-of DNA methyltransferase mutant of Arabidopsis. Combinational analyses of the methylomes and the transcriptomes have indicated that some sense/antisense transcripts are associated with body methylation. I also examined correlation between body methylation and small RNAs.

Research Products

• [Journal Article] Trans-acting small RNA determines dominance relationships in Brassica self-incompatibility (2010)
  • Author(s): Yoshiaki Tarutan, Hiroshi Shiba, Megumi Iwano, Tomohiro Kakizaki, Go Suzuki, Masao Watanabe, Akira Isogai, Seiji Takayama.
  • Journal Title: Nature 466 Pages: 983-986
  • Peer Reviewed
• [Journal Article] Epigenetic control of dominance relationships among self-incompatibilityalleles by DNA methylation. (2010)
  • Author(s): Hiroshi Shiba, Yoshiaki Tarutani, Seiji Takayama.
  • Journal Title: Plant Morphology 22 Pages: 3-8
• [Presentation] トランス作用性small RNAを介したアブラナ科植物でみられる優劣性の制御機構 (2011)
  • Author(s): 樽谷芳明, 柴博史, 岩野恵, 柿崎智博, 鈴木剛, 渡辺正夫, 磯貝彰, 高山誠司
  • Organizer: 日本農芸化学会
  • Place of Presentation: 京都(震災の影響で大会は中止)
  • Year and Date: 2011-03-26
• [Presentation] レーザーマイクロダイセクションを利用したシロイヌナズナ葯タペート組織における遺伝子発現様態の網羅的解析 (2011)
  • Author(s): 木本剛彰, 柴博史, 岩野恵, 藤田雅丈, 高橋宏和, 倉田のり, 中園幹生, 磯貝彰, 高山誠司
  • Organizer: 日本植物生理学会
  • Place of Presentation: 仙台(震災の影響で大会は中止)
  • Year and Date: 2011-03-22
• [Presentation] トランス作用性small RNAによるアブラナ科植物自家不和合性における優劣性の制御 (2011)
  • Author(s): 樽谷芳明, 柴博史, 岩野恵, 柿崎智博, 鈴木剛, 渡辺正夫, 磯貝彰, 高山誠司
  • Organizer: 日本植物生理学会
  • Place of Presentation: 仙台(震災の影響で大会は中止)
  • Year and Date: 2011-03-20
• [Presentation] アブラナ科植物の自家不和合性対立遺伝子でみられるエピジェネティックな遺伝子発現制御 (2010)
  • Author(s): 樽谷芳明, 柴博史, 岩野恵, 柿崎智博, 鈴木剛, 渡辺正夫, 磯貝彰, 高山誠司
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] Regulation of dominance relationships in Brassica self-incompatibility alleles. (2010)
  • Author(s): Yoshiaki Tarutani, Hiroshi Shiba, Megumi Iwano, Tomohiro Kakizaki, Go Suzuki, Masao Watanabe, Akira Isogai, Seiji Takayama.
  • Organizer: Cold Spring Harbor Asia Conference.
  • Place of Presentation: Suzhou (China)
  • Year and Date: 2010-11-02
• [Presentation] アブラナ科植物の自家不和合性でみられるエピジェネティックな遺伝子発現制御 (2010)
  • Author(s): Hiroshi Shiba, Taketo Kayabe, Kentaro Yanol, Junshi Yazaki, Huaming Chen, Yutaka Suzuki, Masahiro Fujita, Nori Kurata, Akira Isogai, Joseph R.Ecker, Seiji Takayama.
  • Organizer: 日本遺伝学会
  • Place of Presentation: 札幌
  • Year and Date: 2010-09-20
• [Presentation] Genome-wide analyses of allele-specific expression and DNA methylation in Arabidopsis thaliana (2010)
  • Author(s): 柴博史, 栢部健人, Kentaro Yanol, 矢崎潤史, Huaming Chen, 鈴木剛, 藤田雅丈, 倉田のり, 磯貝彰, Joseph R.Ecker, 高山誠司
  • Organizer: 21st International Conference on Arabidopsis Research
  • Place of Presentation: Yokohama
  • Year and Date: 2010-06-08
• [Presentation] シロイヌナズナ種内雑種を利用した対立遺伝子間の優劣に関わるDNAメチル化機構の解析 (2010)
  • Author(s): 柴博史, 栢部健人, 樽谷芳明, 藤田雅丈, 倉田のり, 矢崎潤史, Joseph R.Ecker, 磯貝彰, 高山誠司
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-28
• [Presentation] アブラナの自他認識におけるDNAメチル化の役割 (2010)
  • Author(s): 柴博史
  • Organizer: 第18回農芸化学Frontiersシンポジウム
  • Place of Presentation: 千葉
  • Year and Date: 2010-03-27
• [Presentation] (2010)
  • Author(s): 柴博史, 栢部健人, 樽谷芳明, 藤田雅丈, 倉田のり, 矢崎潤史, Joseph R.Ecker, 磯貝彰, 高山誠司
  • Organizer: 第51回日本植物生理学会
  • Place of Presentation: 熊本
  • Year and Date: 2010-03-18
• [Presentation] Regulation of dominance relationships between self-incompatibility alleles in Brassica via de novo DNA methylation. (2010)
  • Author(s): Yoshiaki Tarutani, Hiroshi Shiba, Megumi Iwano, Tomohiro Kakizaki, Masao Watanabe, Akira Isogai, Seiji Takayama.
  • Organizer: International Symposium of Cell-Cell Communicatiion in Plant Reproduction
  • Place of Presentation: Nara
  • Year and Date: 2010-03-11
• [Presentation] Genome-wide analyses of allele-specific expression and DNA methylation in Arabidopsis species. (2010)
  • Author(s): Hiroshi Shiba, Taketo Kayabe, Kentaro Yano, Junshi Yazaki, Huaming Chen, Akira Isogai, Jpseph R. Ecker, Seiji Takayama.
  • Organizer: Keystone Symposia on Molecular and Cellular Biology "RNA Silencing Mechanisms in Plants"
  • Place of Presentation: Santa Fe
  • Year and Date: 2010-02-22
• [Presentation] アブラナ科の自他認識機構～DNAメチル化の役割～ (2009)
  • Author(s): 柴博史
  • Organizer: (財)バイオインダストリー協会 「未来へのバイオ技術」勉強会月例会-植物エピジェネティクスの応用を展望する
  • Place of Presentation: 東京
  • Year and Date: 2009-10-29
• [Presentation] Regulation of dominance relationships between self-incompatibility alleles via de novo DNA methylation. (2009)
  • Author(s): Yoshiaki Tarutani, Hiroshi Shiba, Megumi Iwano, Tomohiro Kakizaki, Masao Watanabe, Akira Isogai, Seiji Takayama.
  • Organizer: 9th International Plant Molecular Biology (IPMB)
  • Place of Presentation: St.Louis
  • Year and Date: 2009-10-28
• [Presentation] シンポジウム「高等植物の生殖および初期発生研究の最前線」S対立遺伝子の発現制御機構の解析からメンデルの優性の法則に迫る (2009)
  • Author(s): 柴博史, 樽谷芳明, 岩野恵, 磯貝彰, 柿崎智博, 渡辺正夫, 高山誠司
  • Organizer: 第73回日本植物学会
  • Place of Presentation: 山形
  • Year and Date: 2009-09-19
• [Presentation] シロイヌナズナで見られるde novo DNAメチル化の網羅的解析 (2009)
  • Author(s): 柴博史, 矢崎潤史, 磯貝彰, Joseph R Ecker, 高山誠司
  • Organizer: 日本農芸化学会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-29