2010 Fiscal Year Final Research Report
Study on an operon rule toward designing of artificial operon
| Project/Area Number |
21710203
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied genomics
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| Research Institution | Keio University |
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Principal Investigator |
TSUGE Kenji Keio University, 政策・メディア研究科, 講師 (70399690)
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| Project Period (FY) |
2009 – 2010
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| Keywords | ゲノムデザイン / オペロン / 遺伝子集積 / 解糖系 / OGAB / 大腸菌 / 枯草菌 |
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| Research Abstract |
To identify possible operon rule, glycolytic genes of Escherichia coli were assembled in multiple types of polycistronic operons that have variety in gene connection order. Through evaluation of functionality by growth and gene expression analysis, a feature of operon in which gene expression profile is downward trend from promoter-proximal to -distal position was revealed. Arrangement of genes according to expression strength of the genes in wild type gave an operon with fastest growth in tested.
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