2010 Fiscal Year Final Research Report
Building of a cassava information resource and understanding of environmental stress responses
| Project/Area Number |
21710205
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied genomics
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
SAKURAI Tetsuya The Institute of Physical and Chemical Research, ゲノム情報統合化ユニット, ユニットリーダー (90415167)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 植物 / 作物 / ゲノム / トランスクリプトーム / 環境応答 / データベース |
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| Research Abstract |
We developed a oligo custom microarray chip which includes approximately 22,000 60-mer probes. The microarray chip was designed by using the ESTs from GenBank (NCBI) and flanking sequences of RIKEN cassava full-length cDNA. Using the microarray chip, we were able to outline gene expression among 3 cassava accession and the 2 stress experimental conditions. Moreover, we performed gene expression profiling via CAGE method, obtained the different gene expression profiles from the 2 methodological aspects.
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