2010 Fiscal Year Final Research Report
Transcriptional regulation analysis and construction of database based on massive 5'-EST sequences
| Project/Area Number |
21710210
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
ゲノム情報科学
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| Research Institution | The University of Tokyo |
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Principal Investigator |
YAMASHITA Riu The University of Tokyo, 医科学研究所, 特任助教 (10401259)
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| Project Period (FY) |
2009 – 2010
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| Keywords | ゲノム / 転写制御 / プロモータ / データベース |
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| Research Abstract |
We have constructed the DataBase of Transcription Start Sites (DBTSS : http://dbtss.hgc.jp/) which contains the information of accurate transcription start sites (TSSs) based on experimentally determined 5'-end clones based on the oligo-capping method. We updated the database, and now it has about 300 million new short sequences. Based on the DBTSS data, we observed significant characteristics difference in major TSSs : namely, highly ordered nucleosome structures, strong RNA polymerase II binding signals, more frequent translation. These results indicate that our database provides a powerful solution not only to discriminate TSCs having clear biological significance from the other possible noise level transcriptions, but also to analyze transcription regulation.
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