2010 Fiscal Year Final Research Report
Design and creation of condition-responsive nano-drug-carriers involving by the step-wise construction method using intelligent nucleic acids
| Project/Area Number |
21710221
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Living organism molecular science
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| Research Institution | Gunma University |
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Principal Investigator |
KUWAHARA Masayasu Gunma University, 大学院・工学研究科, 准教授 (40334130)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 核酸 / 有機化学 / バイオテクノロジー / 生体生命情報学 |
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| Research Abstract |
Intelligent nucleic acids that can release/capture particular drug depending on the condition were created by designing and modifying drug-binding aptamers. Moreover, modified nucleotides were enzymatically incorporated to them by the post-modification method. The incorporation efficiencies were varied significantly depending on sequences around 3'-end of oligonucleotides tethered and chemical structures of modified nucleotides incorporated, which could be improved to some extent, by use of genetically modified polymerases.
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