2010 Fiscal Year Final Research Report
Investigation of plant immune system using a novel elicitor peptide using as a chemical probe, which was identified from random peptide libraries
Project/Area Number |
21710226
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Living organism molecular science
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Research Institution | Kyoto University |
Principal Investigator |
MIYASHITA Masahiro Kyoto University, 大学院・農学研究科, 助教 (80324664)
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Project Period (FY) |
2009 – 2010
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Keywords | ケミカルバイオロジー |
Research Abstract |
Plants defend themselves using an innate immune system, which is activated in response to a variety of elicitor molecules. Previously, we discovered a novel elicitor peptide (YGIHTH-amide, PIP-1) from a combinatorial random library. In this study, we first evaluated the effect of degradation of PIP-1 on defense responses and found that continuous activation is required for phytoalexin biosynthesis. Next, we synthesized a PIP-1 analog, in which the His6 residue is substituted with Cys, to obtain a labeled analog for characterization of its receptor. Modification at the Cys residue using iodophenylmaleimide had no effect on activity, indicating that this analog can be used as a radiolabeled ligand.
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Research Products
(6 results)