2010 Fiscal Year Final Research Report
Development of cell-free synthesis and imaging of membrane proteins in giant vesicles
| Project/Area Number |
21750073
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Analytical chemistry
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
TOYOTA Taro The University of Tokyo, 大学院・総合文化研究科, 講師 (80422377)
|
|---|
| Project Period (FY) |
2009 – 2010
|
| Keywords | 生体分析 / 膜タンパク質 / ベシクル / 反射干渉顕微鏡 |
|---|
| Research Abstract |
The aim of this study is the development of an analytical method to observe the non-equilibrium dynamics of membrane proteins incorporated into model cell membrane. Giant vesicle (GV), which is closed bilayer lipid membrane with cellular size, was used as a reactor of model cell membrane for the cell-free synthesis of a fluorescent membrane protein, eGFP-BmOR1, and the spontaneous membrane insertion of eGFP-BmOR1 synthesized inside of GV was successfully observed. Upon the construction of a reflective interference contrast microscope, the non-equilibrium dynamics of GV settling on a glass substrate was revealed in a nanometer thickness resolution. The combination of these two technologies is expected to promote further progress in the analysis of the non-equilibrium dynamics of membrane proteins and lipid membrane.
|
