2010 Fiscal Year Final Research Report
Fluorescence detection of induction potential on chiral bio-macromolecule under pulsed magnetic field.
| Project/Area Number |
21750076
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Analytical chemistry
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| Research Institution | Osaka University |
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Principal Investigator |
SUWA Masayori Osaka University, 大学院・理学研究科, 助教 (90403097)
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| Project Period (FY) |
2009 – 2010
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| Keywords | パルス磁場 / 誘導起電力 / らせん構造 / キラル認識 / 蛍光検出 |
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| Research Abstract |
We examined to detect the induction potential on a bio-helical structure under pulsed magnetic field by using potential susceptible fluorescence dye. A plasmid DNA, which has supercoiled structure, was coated by Ag nanoparticles to increase the conductivity, and then was bounded with fluorescein. The functionalized plasmid DNA was dispersed in a saline and the fluorescence change accompanied with the pulsed magnetic field was observed. This fluorescence change took place with ～100 μs delay from the applied magnetic field. It was suggested that this is due to the orientation caused by the interaction between the pulsed magnetic field and the induced magnetic momentum in the helical structure.
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