2010 Fiscal Year Final Research Report
Discrimination of methylated nucleobases by a novel modified DNA forming Schiff base
| Project/Area Number |
21750171
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemistry related to living body
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| Research Institution | Osaka University |
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Principal Investigator |
DOHNO Ghikara Osaka University, 産業科学研究所, 准教授 (60420395)
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| Project Period (FY) |
2009 – 2010
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| Keywords | バイオテクノロジー / メチル化核酸塩基 / クロスリンク / DNA / 分子認識 / 有機化学 |
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| Research Abstract |
We have developed a novel chemistry-based method to detect all types of natural DNA methylation. A DNA probe functionalized with a formyl group discriminates between non-methylated and methylated nucleobases, such as 5-methylcytosine, N4-methylcytosine, and N6-methyladenine, by the formation of a selective interstrand cross-link.
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