2010 Fiscal Year Final Research Report
Quantitative analysis of high-order structures of non-coding DNA and non-coding RNA sequences
| Project/Area Number |
21750179
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemistry related to living body
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| Research Institution | Konan University |
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Principal Investigator |
MIYOSHI Daisuke Konan University, フロンティアサイエンス学部, 准教授 (50388758)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 生体機能関連化学 / non-coding RNA / 遺伝子発現 |
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| Research Abstract |
As shown in the on-coding RNAs such as ribozymes and riboswitches, DNA sequences, involving telomeric DNA and untranslated regions which do not posses genetic information, should have regulatory roles in gene expression. Here we investigated structure and thermodynamics of such non-coding DNAs under molecular crowding conditions. It was found that the guanine-rich RNA sequences, derived from telomere sequences, were only able to fold into a parallel-stranded G-quadruplex, and this folding was almost independent of sequence and surrounding factors. In contrast, corresponding DNAs formed various G-quadruplexes depending on the sequence and surrounding factors. These results suggest importance of non-canonical structures of the non-coding DNA sequences which may participate various biological processes.
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