2010 Fiscal Year Final Research Report
Evaluation of adhesivity of blood cells on solid surfaces using the inclined centrifuge microscope
| Project/Area Number |
21760119
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Fluid engineering
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| Research Institution | Tohoku University |
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Principal Investigator |
SHIRAI Atsushi Tohoku University, 流体科学研究所, 准教授 (20302226)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 好中球 / 血管内皮細胞 / 傾斜遠心顕微鏡 / 付着特性 |
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| Research Abstract |
Neutrophils are the most abundant type of leukocytes. It is known that they adhere onto endothelial cells of venules and roll along the vessels slower than the whole blood. In fine capillaries whose inner diameter is smaller than the neutrophils, the cells could plug the vessels to cease blood flow. Therefore, it is essential to understand blood flow characteristics in microvasculature or behavior of neutrophils in immune response that clarifying interaction between the blood cells and the solid surfaces. In this research, we observed motion of HL60 cells, a kind of model cell of neutrophils, on a flat glass plate or cultured endothelial cells using the inclined centrifuge microscope to quantitatively evaluate adhesive characteristics of the blood cells on those solid surfaces.
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