A NADH-dependent fiber-optic biosensor with a UV-LED excitation system

2010 Fiscal Year Final Research Report

• Project/Area Number: 21760310
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Measurement engineering
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: MIYAJIMA Kumiko Tokyo Medical and Dental University, 生体材料工学研究所, 技術職員 (10516298)
• Research Collaborator: MITSUBAYASHI Kohji 東京医科歯科大学, 生体材料工学研究所, 教授 (40307236) ; KUDO Hiroyuki 東京医科歯科大学, 生体材料工学研究所, 講師 (70329118) ; ARAKAWA Takahiro 東京医科歯科大学, 生体材料工学研究所, 助教 (50409637)
• Project Period (FY): 2009 – 2010
• Keywords: バイオセンサ / 高輝度紫外線LED / NADH / アルコール脱水素酵素 / 光ファイバ

Research Abstract

A fiber optic bio-sniffer for high-selective alcohol vapor monitoring with an UV-LED was constructed and tested. In order to achieve high selectivity, the sensor utilizes fluorescence of nicotineamide adenine dinucleotide (NADH), which is produced by the enzymatic reaction of alcohol dehydrogenase. The bio-sniffer was fabricated by attaching a wet enzyme immobilized membrane to the fiber-optic NADH measurement system. The fluorescent intensity significantly increased with a stable value related to the ethanol gas concentration when ethanol vapor was exposed.

Research Products

• [Journal Article] Fluorescence immunoassay using an optical fiber for determination of Dermatophagoides farinae (Der f1) (2011)
  • Author(s): K.Miyajima, G.Itabashi, T.Koshida, K.Tamari, D.Takahashi, T.Arakawa, H.Kudo, H.Saito, K.Yano, K.Shiba, K.Mitsubayashi
  • Journal Title: Environmental Monitoring and Assessment in press
• [Journal Article] Biosensor for L-phenylalanine based on the optical detection of NADH using a UV light emitting diode (2011)
  • Author(s): T.Arakawa, T.Koshida, T.Gessei, K.Miyajima, D.Takahashi, H.Kudo, K.Yano, K.Mitsubayashi
  • Journal Title: Microchimica Acta 173 Pages: 199-205
• [Journal Article] Soft contact lens biosensor for in-situ monitoring of tear glucose as non-invasive blood sugar assessment (2011)
  • Author(s): M.X.Chu, K.Miyajima, D.Takahashi, T.Arakawa, K.Sano, S.Sawada, H.Kudo, Y.Iwasaki, K.Akiyoshi, M.Mochizuki, K.Mitsubayashi
  • Journal Title: Talanta 83 Pages: 960-965
• [Journal Article] Flexible humidity sensor in a sandwich configuration with a hydrophilic porous membrane (2009)
  • Author(s): Y.Miyoshi, K.Miyajima, H.Saito, H.Kudo, T.Takeuchi, I.Karube, K.Mitsubayashi
  • Journal Title: Sensors and Actuators B 142 Pages: 28-32
• [Journal Article] A NADH-dependent alcohol gas sensor (bio-sniffer) with an UV-LED (2009)
  • Author(s): H.Ishimaru, H.Kudo, Y.Suzuki, T.Gessei, K.Miyajima, H.Saito, K.Mitsubayashi
  • Journal Title: Journal of Advanced Science 21 Pages: 41-42
• [Journal Article] A NADH-dependent fiber-optic biosensor for ethanol determination with a UV-LED excitation system (2009)
  • Author(s): H.Kudo, M.Sawai, X.Wang, T.Gessei, T.Koshida, K.Miyajima, H.Saito, K.Mitsubayashi
  • Journal Title: Sensors and Actuators B 141 Pages: 20-25
• [Journal Article] A soft and flexible biosensor using a phospholipid polymer for continuous glucose monitoring (2009)
  • Author(s): M.X.Chu, H.Kudo, T.Shirai, K.Miyajima, H.Saito, N.Morimoto, K.Yano, Y.Iwasaki, K.Akiyoshi, K.Mitsubayashi
  • Journal Title: Biomedical Microdevices 11 Pages: 837-842
• [Presentation] アルコール代謝評価のための呼気中エタノールガス可視化システム (2011)
  • Author(s): 芳賀俊介, 北和昂, 王シン, 宮島久美子, 高橋大志, 荒川貴博, 工藤寛之, 三林浩二
  • Organizer: 第58回応用物理学関係連合講演会
  • Place of Presentation: 厚木
  • Year and Date: 20110300
• [Presentation] 住環境中ホルムアルデヒドの高感度モニタリングを目的とした生化学式ガスセンサ (2010)
  • Author(s): 山下俊文, 板橋玄, 月精智子, 宮島久美子, 高橋大志, 荒川貴博, 工藤寛之, 三林浩二
  • Organizer: 第22回SASインテリジェントシンポジウム
  • Place of Presentation: 平塚
  • Year and Date: 20101100
• [Presentation] Fiber-optic fluoro- immunoassay for on-site detection of Dermatphagoides farinae allergen in residential environment (2010)
  • Author(s): K. Miyajima, G. Itabashi, D. Takahashi, T. Arakawa, H. Kudo, H. Saito, K. Mitsubayashi
  • Organizer: 13th Internationla Meeting on Chemical Sensors
  • Place of Presentation: Perth
  • Year and Date: 20100700
• [Presentation] ダニアレルゲンDer f1の住環境における動態評価のための蛍光免疫計測に関する研究 (2010)
  • Author(s): 宮島久美子, 板橋玄, 玉利佳子, 高橋大志, 荒川貴博, 工藤寛之, 齊藤浩一, 矢野和義, 芝紀代子, 三林浩二q
  • Organizer: 第71回分析化学討論会
  • Place of Presentation: 松江
  • Year and Date: 20100500
• [Presentation] A high-sensitive fiber- optic biosensor for Phenylalanine using an UV-LED excitation system (2009)
  • Author(s): G. Itabashi, T. Koshida, K. Miyajima, D. Takahashi, T. Arakawa, H. Kudo, K. Mitsubayashi
  • Organizer: 8th Asian Conference on Chemical Sensors
  • Place of Presentation: Daegu
  • Year and Date: 20091100
• [Presentation] Fiber-optic fluoro- immunoassay system for a determination of mite allergen in residential atmosphere (2009)
  • Author(s): K. Miyajima, G. Itabashi, T. Koshida, D. Takahashi, T. Arakawa, H. Saito, H. Kudo, K. Mitsubayashi
  • Organizer: 8th Asian Conference on Chemical Sensors
  • Place of Presentation: Daegu
  • Year and Date: 20091100
• [Presentation] Fiber optic Bio-sniffer (biochemical gas sensor) using UV-LED light for monitoring ethanol vapor with high sensitivity & selectivity (2009)
  • Author(s): H. Kudo, K. Miyajima, D. Takahashi, T. Arakawa, H. Saito, K. Mitsubayashi
  • Organizer: The 8th annual IEEE Conference on Sensors, Christchurch
  • Place of Presentation: Daegu
  • Year and Date: 20091000
• [Presentation] Optical Bio-sniffer (gas sensor) using UV-LED for gaseous ethanol (2009)
  • Author(s): M. Sawai, K. Miyajima, D. Takahashi, T. Arakawa, H. Saito, H. Kudo, K. Mitsubayashi
  • Organizer: 6th International Conference Instrumental Methods of Analysis Modern Trends and Applications
  • Place of Presentation: Athens
  • Year and Date: 20091000
• [Presentation] A flow analysis of E.coli O157:H7 by fiber optic fluorescent immunoprobe (2009)
  • Author(s): T. Koshida, M. Taniguchi, K. Miyajima, D. Takahashi, T. Arakawa, H. Kudo, K. Yano,, K. Mitsubayashi
  • Organizer: FLOW ANALYSIS XI
  • Place of Presentation: Mallorca
  • Year and Date: 20090900
• [Presentation] 住環境における高感度VOC計測をめざした生化学式ガスセンサ (2009)
  • Author(s): 工藤寛之, 鈴木祐貴, 月精智子, 宮島久美子, 高橋大志, 荒川貴博, 齊藤浩一, 三林浩二
  • Organizer: 電気学会 バイオ・マイクロシステム研究会
  • Place of Presentation: 東京
  • Year and Date: 20090700
• [Presentation] Biochemical gas sensor (Bio-sniffer) using an ultraviolet LED for ethanol gas measurement (2009)
  • Author(s): H. Kudo, M. Sawai, T. Gessei, K. Miyajima, D. Takahashi, T. Arakawa, H. Saito, K. Mitsubayashi
  • Organizer: 9th Workshop on (Bio)sensors and Bioanalytical microtechniques in environmental and clinical analysis
  • Place of Presentation: Montreal
  • Year and Date: 20090600
• [Presentation] ダニアレルゲンDer f I測定のための光ファイバ式蛍光免疫計測システムに関する研究 (2009)
  • Author(s): 宮島久美子, 越田智之, 齊藤浩一, 工藤寛之, 三林浩二
  • Organizer: 第48回日本生体医工学会大会
  • Place of Presentation: 東京
  • Year and Date: 20090400
• [Book] 展望とトピックス(光ファイバー技術を利用してダニアレルゲンをリアルタイムで測定,第71回分析化学討論会) (2010)
  • Author(s): 宮島久美子, 板橋玄, 玉利佳子, 高橋大志, 荒川貴博, 工藤寛之, 齊藤浩一, 矢野和義, 芝紀代子, 三林浩二
  • Publisher: 社団法人 日本分析化学会 展望とトピックス委員会(東京)
• [Book] ヘルスケアのための環境バイオセンサ,ヘルスケアとバイオ医療のための先端デバイス機器(三林浩二 監修)(第34章) (2009)
  • Author(s): 宮島久美子, 齊藤浩一, 三林浩二
  • Publisher: シーエムシー出版(東京)