Development of monomer-supplying enzymes for cell-free biopolyester production

2010 Fiscal Year Final Research Report

• Project/Area Number: 21760632
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biofunction/Bioprocess
• Research Institution: Hokkaido University
• Principal Investigator: SATOH Yasuharu Hokkaido University, 大学院・工学研究院, 助教 (30360928)
• Research Collaborator: TAJIMA Kenji 北海道大学, 大学院・工学研究院, 准教授 (00271643) ; HAN Xuerong 北海道大学, 大学院・工学研究院, 博士後期課程
• Project Period (FY): 2009 – 2010
• Keywords: 酵素反応 / 環境材料 / バイオテクノロジー / バイオリアクター

Research Abstract

Polyhydroxyalkanoates (PHAs), which originate from renewable resources such as sugars and vegetable oils, have attracted great interest as alternatives to petrochemical-based plastics. To construct a novel cell-free PHA production system from sugars as feedstocks, an (R)-specific and NADH-dependent reductase is needed. In this study, we successfully developed the reductases to exchange stereo-selectivity of an (S)-specific enzyme. In addition, we could find the reductase from databases.

Research Products

• [Journal Article] Chemo-enzymatic synthesis of polyhydroxyalkanoate by an improved two-phase reaction system (TPRS) (2009)
  • Author(s): Xuerong Han,Yasuharu Satoh, Kenji Tajima, Tokuo Matsushima, Masanobu Munekata
  • Journal Title: Journal of Bioscience and Bioengineering 108 Pages: 517-523
  • Peer Reviewed
• [Journal Article] 微生物を活用したバイオポリマーへの物質変換 (2009)
  • Author(s): 佐藤康治, 田島健次, 松島得雄, 大嶋武, 棟方正信
  • Journal Title: 環境技術 38 Pages: 14-18
• [Journal Article] Chemo-enzymatic synthesis of polyhydroxyalkanoate (PHA) incorporating 2-hydroxybutyrate by wild-type Class I PHA synthase from Ralstonia eutropha
  • Author(s): Xuerong Han, Yasuharu Satoh, Toshifumi Satoh, Ken'ichiro Matsumoto, Toyoji Kakuchi, Seiichi Taguchi, Tohru Dairi, Masanobu Munekata, Kenji Tajima,
  • Journal Title: Applied Microbiology and Biotechnology in press
  • Peer Reviewed
• [Presentation] 耐熱性PHA合成細菌由来PHA合成酵素遺伝子のクローニングとその解析 (2011)
  • Author(s): 佐藤康治、松谷枝里子、韓雪容、中本翔、田島健次、松島得雄、棟方正信
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都
  • Year and Date: 20110326-20110328
• [Presentation] 改良水-有機溶媒二相反応系による新規ポリヒドロキシアルカン酸の合成 (2010)
  • Author(s): 韓雪容, 佐藤康治, 田島健次, 鳥谷部哲也, 松島得雄, 大利徹, 棟方正信
  • Organizer: 高分子学会第59回高分子討論会
  • Place of Presentation: 札幌
  • Year and Date: 20100915-20100917
• [Presentation] 官能基修飾によるポリヒドロキシアルカン酸の高機能化 (2010)
  • Author(s): 岩本浩介, 谷尾亮, 佐藤康治, 田島健次, 堺井亮介, 佐藤敏文, 鳥谷部哲也, 松島得雄, 覚知豊治, 大利徹, 棟方正信
  • Organizer: 高分子学会第59回高分子討論会
  • Place of Presentation: 札幌
  • Year and Date: 20100915-20100917
• [Presentation] One-potポリヒドロキシアルカン酸合成法の開発 (2009)
  • Author(s): 佐藤康治
  • Organizer: バイオポリマー研究の最前線-バイオと化学のクロストーク-
  • Place of Presentation: 札幌
  • Year and Date: 2009-06-29