2010 Fiscal Year Final Research Report
PCNA-based P450-electron transfer proteins complex
| Project/Area Number |
21760633
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | The University of Tokyo |
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Principal Investigator |
HIRAKAWA Hidehiko The University of Tokyo, 大学院・工学系研究科, 助教 (90451799)
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| Project Period (FY) |
2009 – 2010
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| Keywords | シトクロムP450 / 電子伝達タンパク質 / ヘテロ3量体 / 古細菌 / PCNA |
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| Research Abstract |
Bacterial cytochrome P450s require electrons for its catalytic activities through two kinds of electron transfer proteins. To improve electron transfer efficiency, a protein complex composed of cytochrome P450 and its electron transfer proteins was constructed using a heterotimeric protein. Its catalytic activity was evaluated and the component fusion proteins were optimized. E.coli harboring the complex was examined to use as a biocatalyst.
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