2010 Fiscal Year Final Research Report
Regulating cell function by introduction of the proteins enzymatically modified with synthetic functional molecules into cell membrane or cytoplasm
| Project/Area Number |
21760634
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | The University of Tokyo |
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Principal Investigator |
YAMAGUCHI Satoshi The University of Tokyo, 大学院・工学系研究科, 助教 (80398106)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 蛋白質 / 細胞内導入 / 酵素 / 細胞免疫療法 / 癌 / 抗体 / 膜透過性ペプチド |
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| Research Abstract |
We developed technologies that can transfer target proteins into cellular membrane and cytoplasm by enzymatically-specific modification of the C-terminal of target proteins with synthetic functional molecules. By the present technologies、target proteins could be modified with PEG-lipid on cellular membrane. This method realized that the efficiency in the phagocytosis of dendritic cells for cancer cells was successfully enhanced by introducing antibodies onto cancer membranes. In addition、we developed a method that can easily transfer target proteins to cytoplasm by attaching cell-penetrating peptide bearing the dye、which generates radical under photo-irradiation、to target protein and irradiating light after cellular uptake of the present modified protein.
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