2010 Fiscal Year Final Research Report
Development of techniques for membrane protein assembly into nano vesicles using structural proteins
| Project/Area Number |
21760635
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
YOSHINO Tomoko Tokyo University of Agriculture and Technology, 大学院・工学研究院, 特任准教授 (30409750)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 小胞 / 構造タンパク質 / 細胞 / 遺伝子融合 |
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| Research Abstract |
In this study, the techniques for production of "target molecule-assembled nano vesicles" using structural proteins have been developed. W succeeded in forming vesicles and integrating proteins onto the vesicles using Mms13, which was isolated as a protein tightly bound to bacterial magnetic particles. Furthermore, the method for purification of the vesicles form cell homogenates have been established, and a novel tool was developed toward the analysis of target molecules.
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