2010 Fiscal Year Final Research Report

Eiucidation of the mobilization mechanism of transposab leel ements

Research Project

Project/Area Number	21770002
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Genetics/Genome dynamics
Research Institution	Tokyo Institute of Technology
Principal Investigator	KAJIKAWA Masaki Tokyo Institute of Technology, 大学院・生命理研究科講師生命理工学研究科, 講師 (90361766)
Project Period (FY)	2009 – 2010
Keywords	転移因子 / レトロトランスポゾン / 反復配列
Research Abstract	Long interspersed elements (LINEs) are transposable elements that mobilize and amplify their own copies within eukaryotic genomes. LINEs mobilize via a mechanism called retrotransposition, in which transcribed LINE RNA is reverse transcribed into DNA that is then integrated into the host chromosome. Although the role of LINE-encoded proteins in retrotransposition has been revealed, the participation of host-encoded proteins has not been well investigated. Here, we present genetic evidence that the host-encoded proteins involved in repair of DNA double-strand breaks participate in LINE retrotransposition.