2010 Fiscal Year Final Research Report
Analysis of clock genes and proteins in Chlamydomonas: a new model system for studies on the circadian clock.
| Project/Area Number |
21770004
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Genetics/Genome dynamics
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| Research Institution | Nagoya University |
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Principal Investigator |
MATSUO Takuya Nagoya University, 遺伝子実験施設, 助教 (00452201)
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| Project Period (FY) |
2009 – 2010
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| Keywords | モデル生物 / 生物時計 |
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| Research Abstract |
We analyzed clock genes and proteins in the unicellular green algae Chlamydomonas reinhardtii that is a new model system for circadian clock research. We revealed a genetic interaction between ROC75 and ROC40 through experiments of overexpression and RNA interference of ROC75 gene. Moreover, We expressed ROC75 protein tagged with HA, YFP, and LUC in Chlamydomonas, and revealed that the ROC75 protein level is regulated by the circadian clock, and is localized into the nuclear periphery. In an in vitro and in vivo assay, we demonstrated that ROC75 binds to the promoter region of the ROC40 gene. These results suggest that ROC75 is a transcriptional regulator for ROC40 gene, and the regulation may be important for the circadian clock mechanism in Chlamydomonas. These findings will provide new insights into the evolution of circadian clocks in the green plant lineage.
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