2011 Fiscal Year Final Research Report
Elucidation of ICE1-dependent cold signaling network
| Project/Area Number |
21770032
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
MIURA Kenji 筑波大学, 生命環境系, 助教 (00507949)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 低温シグナル伝達 / ICE1の安定化 / カルモジュリン様タンパク質 |
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| Research Abstract |
To identify the transactivation system of ICE1, which is a regulator of cold signaling, site-directed mutagenesis was introduced. Substitution of serine 403 to alanine inhibited degradation of ICE1, consequently, ICE1(S403A) was stabilized. This stabilization leads to improving cold tolerance. And to understand the regulatory mechanisms of ICE1, interactors of ICE1 were isolated by yeast 2-hybrid screening. MYC-type transcription factors, kinases, and calmodulin-like proteins were identified as ICE1-interactors. Furthermore, the results suggested that calmodulin-like proteins are involved in regulation of cold signaling and cold tolerance.
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