Analysis of novel defense response system in plant by AtNFXL1 through digestion of protein

2010 Fiscal Year Final Research Report

• Project/Area Number: 21770038
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kanazawa University
• Principal Investigator: ASANO Tomoya Kanazawa University, 学際科学実験センター, 博士研究員 (00377409)
• Project Period (FY): 2009 – 2010
• Keywords: シロイヌナズナ / AtNFXL1 / MKD1トリコテセン / 病害抵抗性 / ムギ類赤かび病菌

Research Abstract

AtNFXL1 protein is digested on Zn finger domain, and acts as two proteins of N80 and C62 in nuclear and cytosol, respectively. Furthermore, AtNFXL1 proteins acts as negative regulator, all of domains on AtNFXL1 protein plays important role to resistance of Fusarium species. N80 of AtNFXL1 protein acts as transcription factor. On the other hand, C62 is interact with MAPKKK, and regulate novel MAP kinase cascade.

Research Products

• [Journal Article] Gene Expression Analysis ofWounding-Induced Root-to-Shoot Communication in Arabidopsis thaliana (2011)
  • Author(s): Hasegawa, S., Sogabe, Y., Asano, T.,Nakagawa, T., Nakamura, H., Kodama, H.,Ohta, H., Yamaguchi, K., Mueller, M., J., Nishiuchi, T.
  • Journal Title: Plant Cell and Environmental 34 Pages: 705-716
  • Peer Reviewed
• [Journal Article] Visualization of Wound-induced Root-to-Shoot Communication in Arabidopsis
  • Author(s): Sogabe, Y.,Nakamura, H., Nakagawa, T.,Hasegawa, S., Asano, T., Ohta, H.,Yamaguchi, K., Mueller, M., J., Kodama H., Nishiuchi., T.
  • Journal Title: Plant Signaling Behavior in press
  • Peer Reviewed
• [Presentation] A novel MAP kinase cascadein Arabidopsis plays a crucial role in diseaseresistance to Fusarium sporotrichioides (2010)
  • Author(s): Asano, T., Mizuno, H., Yamaguchi, K.andNishiuchi, T.
  • Organizer: Plant Biology 2010
  • Place of Presentation: Palais desCongress de Montreal (Canada)
  • Year and Date: 2010-08-03
• [Presentation] Arabidopsis plants aresusceptible to Fusarium sporotrichioidesproducing type A trichothecene phytotoxins (2010)
  • Author(s): Asano, T., Mizuno, H., Yamaguchi, K.andNishiuchi, T.
  • Organizer: The 20th International Conference on PlantGrowth Substances
  • Place of Presentation: Palau de Congressos de Tarragona (Spain)
  • Year and Date: 2010-07-30
• [Presentation] Arabidopsisplants are susceptible to Fusariumsporotrichioides producing type Atrichothecene phytotoxins (2010)
  • Author(s): Asano, T., Sasaki, R., Kato, T., Kimura, M.,Koga H., Nishiuchi, T.
  • Organizer: 21st InternationalConference on Arabidopsis Research
  • Place of Presentation: Pacifico Yokohama (KanagawaPrefecture)
  • Year and Date: 2010-06-07
• [Presentation] トリコテセン産生菌Fusarium sporotrichioidesに対して病害抵抗性因子として機能するArabidopsisthalianaの新規MAPKKKの機能解析 (2010)
  • Author(s): 浅野智哉, 水野宏美, 幸節健, 町田泰則,山口和男, 西内巧
  • Organizer: 第51回日本植物生理学会
  • Place of Presentation: 熊本大学(熊本県)
  • Year and Date: 2010-03-18
• [Presentation] Functional analysis of a novel MAPKKKinduced by Fusarium phytotoxintrichothecenes (2009)
  • Author(s): Asano, T., Mizuno, H., Kosetsu, K., Machida,Y., Yamaguchi, K., Nishiuchi, T.
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2009-12-10
• [Presentation] Functional analysis of a novelMAPKKK induced by Fusarium phytotoxintrichothecenes (2009)
  • Author(s): Asano, T., Mizuno, H., Yamaguchi, K.andNishiuchi, T.
  • Organizer: Plant biology
  • Place of Presentation: Hawaii Convention Center (USA)
  • Year and Date: 2009-07-19
• [Book] Methods in Molecular Biology p225-233 (2011)
  • Author(s): Asano, T., Nishiuchi, T.
  • Publisher: Humana press
• [Remarks] ホームページ等
  • URL: http://web.kanazawa-u.ac.jp/~gene/
• [Patent(Industrial Property Rights)] 赤かび病抵抗性植物の作製方法およびその利用 (2011)
  • Inventor(s): 西内巧、浅野智哉、加藤智朗
  • Industrial Property Rights Holder: 国立大学法人 金沢大学
  • Industrial Property Number: 特許,特願2011-15302
  • Filing Date: 2011-01-27