Characterization of the plastidial pyruvate transporting mechanism

2010 Fiscal Year Final Research Report

• Project/Area Number: 21770048
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Hiroshima University
• Principal Investigator: FURUMOTO Tsuyoshi Hiroshima University, 大学院・理学研究科, 准教授 (30313208)
• Project Period (FY): 2009 – 2010
• Keywords: C4光合成 / フラベリア

Research Abstract

Pyruvate is an essential metabolite for multiple plastid-localized metabolic pathways of plants, including the C4 photosynthetic CO, -concentrating mechanism. Despite the crucial role of pyruvate import into plastids, the molecule mediating pyruvate transport is still unknown. Using differential transcriptome analyses of the C3 and C4 plants of the genus Flaveria, we have identified a novel plastid-localized transporter gene, named BASS2, for bile acid : sodium symporter family protein 2, the transcript of which is abundant in C4 plants that have the sodium-dependent pyruvate transporter. Recombinant BASS2 shows sodium-dependent pyruvate uptake activity.

Research Products

• [Journal Article] Identification of a plastidial sodium-dependent pyruvate transporter (2011)
  • Author(s): Tsuyoshi Furumoto, Teppei Yamaguchi, Yumiko Ohshima-Ichie, Masayoshi Nakamura, Yoshiko Iwata, Masaki Shimamura, Junichi Ohnishi, Shingo Hata, Udo Gowik, Peter Westhoff, Andrea Brautigam, Andreas P.M.Weber, Katsura Izui
  • Journal Title: Nature in press
  • Peer Reviewed
• [Presentation] Identification of a plastid Na+ dependent pyruvate transport system (2010)
  • Author(s): Furumoto T
  • Organizer: Carbon Concentrating Mechanism
  • Place of Presentation: Awaji, Japan
  • Year and Date: 20100829-20100902
• [Presentation] Identification of a plastid Na+ dependent pyruvate transport system in plants (2010)
  • Author(s): Furumoto T
  • Organizer: Symposium on C4 Plant Biology
  • Place of Presentation: Shanghai, China
  • Year and Date: 20100818-20100820
• [Presentation] C4回路で機能するナトリウム依存性ピルビン酸輸送機構の解明」 (2010)
  • Author(s): 古本強
  • Organizer: 第1回日本光合成学会公開シンポジウム「光合成のダイナミックス」-太陽光エネルギー利用の新しい試み-
  • Place of Presentation: 東京大学駒場
  • Year and Date: 2010-06-04
• [Presentation] Identification of a plastid Na+ dependent pyruvate transporter in plants, functioning C4 pathway and MEP pathway. (2009)
  • Author(s): Furumoto T
  • Organizer: 理化学研究所セミナー
  • Place of Presentation: 理化学研究所
  • Year and Date: 2009-10-23
• [Remarks] ホームページ等