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2011 Fiscal Year Final Research Report

Symbiosis-related genes of the nitrogen-fixing bacterium Frankia

Research Project

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Project/Area Number 21770053
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Plant molecular biology/Plant physiology
Research InstitutionKagoshima University

Principal Investigator

KUCHO Ken-ichi  鹿児島大学, 大学院・理工学研究科, 准教授 (70404473)

Project Period (FY) 2009 – 2011
Keywords共生 / 窒素固定 / 形質転換 / マイクロアレイ
Research Abstract

Frankia is a nitrogen-fixing bacterium having an ability to make symbiosis with trees. Symbiosis occurs in nodules formed on roots in which Frankia fixes nitrogen and supplies the products to the host plant to promote its growth. In order to identify Frankia genes involved in the symbiosis, I attempted to establish transformation system of Frankia and globally identified Frankia genes induced in nodule.

  • Research Products

    (19 results)

All 2012 2011 2010 2009

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (14 results) Book (1 results)

  • [Journal Article] The determinants of the actinorhizal symbiosis2010

    • Author(s)
      Kucho K, Hay A-E and Normand P.
    • Journal Title

      Microbes Environ

      Volume: 25 Pages: 241-252

    • DOI

      doi:10.1264/jsme2.ME10143

    • Peer Reviewed
  • [Journal Article] The Frankia alni symbiotic transcriptome2010

    • Author(s)
      Alloisio N, Queiroux C, Fournier P, Pujic P, Normand P, Vallenet D, Medigue C, Yamaura M, Kakoi K and Kucho K.
    • Journal Title

      Mol Plant Microbe Interact

      Volume: 23 Pages: 593-607

    • DOI

      doi:10.1094/MPMI-23-5-0593

    • Peer Reviewed
  • [Journal Article] Identification by suppression subtractive hybridization of Frankia genes induced under nitrogen-fixing conditions2010

    • Author(s)
      Yamaura M, Uchiumi T, Higashi S, Abe M, Kucho K.
    • Journal Title

      Appl Environ Microbiol

      Volume: 76 Pages: 1962-1964

    • DOI

      doi:10.1128/AEM.01813-09

    • Peer Reviewed
  • [Journal Article] Transient transformation of Frankia by fusion marker genes in liquid culture2009

    • Author(s)
      Kucho K, Kakoi K, Yamaura M, Higashi S, Uchiumi T and Abe M.
    • Journal Title

      Microbes Environ

      Volume: 24 Pages: 231-240

    • Peer Reviewed
  • [Presentation] 樹木と共生する窒素固定放線菌フランキアのゲノム解析2011

    • Author(s)
      九町健一
    • Organizer
      科学技術交流財団研究会「ハイスループットスクーニングと次世代DNAシーケンサーの相乗的応用」
    • Place of Presentation
      愛知
    • Year and Date
      2011-11-11
  • [Presentation] プラスミドを用いたフランキアの形質転換2011

    • Author(s)
      松井勇磨, 栫健太郎, Peter Pujic, 東四郎, 阿部美紀子, Philippe Normand, 内海俊樹, 九町健一
    • Organizer
      植物微生物研究会第21回研究交流会
    • Place of Presentation
      岡山
    • Year and Date
      2011-09-20
  • [Presentation] Isolation of uracil auxotrophic mutants in Frankia2010

    • Author(s)
      Yamaura M, Uchiumi T, Higashi S, Abe M and Kucho K.
    • Organizer
      1st Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
    • Place of Presentation
      Miyazaki
    • Year and Date
      2010-09-20
  • [Presentation] Symbiotic transcriptome of the nitrogen-fixing actinobacterium Frankia2010

    • Author(s)
      Kucho K, Alloisio N, Queiroux C, Fournier P, Pujic P, Yamaura M, Kakoi, K, Uchiumi T, Abe M and Normand P.
    • Organizer
      1st Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
    • Place of Presentation
      Miyazaki
    • Year and Date
      2010-09-20
  • [Presentation] Isolation of uracil auxotrophic mutants in Frankia2010

    • Author(s)
      Yamaura M, Uchiumi T, Higashi S, Abe M and Kucho K.
    • Organizer
      16th International Meeting on Frankia and Actinorhizal Plants
    • Place of Presentation
      ポルト
    • Year and Date
      2010-09-06
  • [Presentation] Use of high-activity promoters and a codon-optimized marker gene for Frankia transformation2010

    • Author(s)
      Yamaura M, Higashi S, Uchiumi T, Abe M and Kucho K.
    • Organizer
      16th International Meeting on Frankia and Actinorhizal Plants
    • Place of Presentation
      ポルト
    • Year and Date
      2010-09-06
  • [Presentation] Recent trials for Frankia transformation2010

    • Author(s)
      Yamaura M, Kakoi K, Iwashita M, Abe M, Uchiumi T and Kucho K.
    • Organizer
      16th International Meeting on Frankia and Actinorhizal Plants
    • Place of Presentation
      ポルト
    • Year and Date
      2010-09-06
  • [Presentation] 樹木と共生窒素固定を行う放線菌フランキアのトランスクリプトーム解析2010

    • Author(s)
      九町健一、Alloisio N、Queiroux C、Fournier P、Pujic P、Vallenet D、Medigue C、山浦真稔、栫健太郎、内海俊樹、阿部美紀子、Normand P.
    • Organizer
      日本植物生理学会第51回大会
    • Place of Presentation
      熊本
    • Year and Date
      2010-03-19
  • [Presentation] Nitrogen-fixing Symbiosis between the Actinobacterium Frankia and Trees2010

    • Author(s)
      Kucho K.
    • Organizer
      7th Okazaki Biology Conferences
    • Place of Presentation
      静岡
    • Year and Date
      2010-01-13
  • [Presentation] 共生窒素固定細菌Frankiaの形質転換法への高発現プロモーターの利用2009

    • Author(s)
      栫健太郎、山浦真稔、東四郎、内海俊樹、阿部美紀子、九町健一
    • Organizer
      植物微生物研究会第19回研究交流会
    • Place of Presentation
      長野
    • Year and Date
      2009-09-20
  • [Presentation] Transformation of Frankia sp. strain CcI3 by fusion marker genes using liquid culture selection2009

    • Author(s)
      Kucho K, Kakoi K, Yamaura M, Higashi S, Uchiumi T, Abe M.
    • Organizer
      XIV International Congress on Molecular Plant-Microbe Interactions
    • Place of Presentation
      ケベック
    • Year and Date
      2009-07-21
  • [Presentation] 共生窒素固定細菌フランキアの形質転換法の確立2009

    • Author(s)
      九町健一、栫健太郎、山浦真稔、内海俊樹、阿部美紀子
    • Organizer
      2009年度日本放線菌学会大会
    • Place of Presentation
      秋田
    • Year and Date
      2009-07-17
  • [Presentation] 共生窒素固定細菌Frankiaの形質転換法への高発現プロモーターの利用2009

    • Author(s)
      栫健太郎、山浦真稔、東四郎、内海俊樹、阿部美紀子、九町健一
    • Organizer
      第24回日本放線菌学会
    • Place of Presentation
      秋田
    • Year and Date
      2009-07-17
  • [Presentation] Suppression subtractive hybtidization法の細菌への応用単生で窒素固定を行うFrankiaで特異的に誘導される遺伝子の探索2009年度2009

    • Author(s)
      山浦真稔、東四郎、内海俊樹、阿部美紀子、九町健一
    • Organizer
      日本放線菌学会大会
    • Place of Presentation
      秋田
    • Year and Date
      2009-07-17
  • [Book] 土壌微生物実験法2012

    • Author(s)
      九町健一、笹川英夫
    • Publisher
      養賢堂(印刷中)

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Published: 2013-07-31  

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