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2010 Fiscal Year Final Research Report

Analysis of Functional Differentiation in the Endoplasmic Reticulum during the ER Body Formation

Research Project

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Project/Area Number 21770057
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Plant molecular biology/Plant physiology
Research InstitutionNational Institute for Basic Biology

Principal Investigator

YAMADA Kenji  基礎生物学研究所, 高次細胞機構研究部門, 助教 (00360339)

Project Period (FY) 2009 – 2010
Keywords小胞体 / ERボディ / シロイヌナズナ / βグルコシダーゼ / 傷害 / 膜タンパク質 / オルガネラ / 防御機構
Research Abstract

Endoplasmic reticulum (ER) body is an ER derived structure that is found in the epidermal cells of Arabidopsis seedlings. ER body is involved in plant defense system against insect and pathogen attack, since wound treatment induced ER bodies. Two ER body proteins, NAI2 and PYK10 are involved in ER body formation, but its function was obscure. To understand the ER body formation in detail, I analyzed ER body proteins in Arabidopsis. I found that two ER body proteins, MEB1 and MEB2 are localized specifically in ER body membrane. Furthermore, I found that NAI2 and PYK10 are necessary and sufficient for the ER body formation. These findings unrevealed unique mechanisms for ER body formation in plants.

  • Research Products

    (14 results)

All 2011 2010 2009 Other

All Journal Article (3 results) (of which Peer Reviewed: 2 results) Presentation (8 results) Remarks (1 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 1 results)

  • [Journal Article] Unique defense strategy by the endoplasmic reticulum body in plants.2011

    • Author(s)
      K. Yamada, I. Hara-Nishimura, M. Nishimura
    • Journal Title

      Plant Cell Physiol.

      Volume: 52(12) Pages: 2039-2049

    • Peer Reviewed
  • [Journal Article] The ER body, a new organelle in Arabidopsis thaliana, requires NAI2 for its formation and accumulates specific β-glucosidases2009

    • Author(s)
      K. Yamada, A. J. Nagano, K. Ogasawara, I. Hara-Nishimura, M. Nishmura
    • Journal Title

      Plant Signal. Behav.

      Volume: 4(9) Pages: 849-852

  • [Journal Article] Constitutive and inducible ER bodies of Arabidopsis thaliana accumulate distinct β-glucosidases2009

    • Author(s)
      K. Ogasawara, K. Yamada, J. T. Christeller, M. Kondo, N. Hatsugai, I. Hara-Nishimura, M. Nishimura
    • Journal Title

      Plant Cell Physiol.

      Volume: 50(3) Pages: 480-488

    • Peer Reviewed
  • [Presentation] NAI2によるERボディ成分の集積はERボディ形成に必要かつ十分である2011

    • Author(s)
      山田健志, 永野惇, 仁科桃子, 西村いくこ, 西村幹夫
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      東北大学, 仙台
    • Year and Date
      20110320-22
  • [Presentation] Mechanisms of ER body formation and its possible function in Arabidopsis thaliana2010

    • Author(s)
      K. Yamada
    • Organizer
      The 2nd NIBB-MPIZ Joint Symposium, Plant Science Communications 2010
    • Place of Presentation
      Okazaki Conference Center, Okazaki, Japan
    • Year and Date
      20101116-18
  • [Presentation] Expression of NAI2 and PYK10 is sufficient for the endoplasmic reticulum (ER) body formation in Arabidopsis2010

    • Author(s)
      K. Yamada, A. J. Nagano, M. Nishina, I. Hara-Nishimura, M. Nishimura
    • Organizer
      21st International Conference on Arabidopsis Research
    • Place of Presentation
      Pacifico Yokohama, Yokohama, Japan
    • Year and Date
      20100606-10
  • [Presentation] NAI2とPYK10はERボディの形成とERボディ膜タンパク質の局在を制御する2010

    • Author(s)
      山田健志, 永野惇, 仁科桃子, 西村いくこ, 西村幹夫
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本大学, 熊本
    • Year and Date
      20100318-21
  • [Presentation] Molecular mechanisms of endoplasmic reticulum body formation in Arabidopsis2010

    • Author(s)
      K. Yamada
    • Organizer
      JSPS Invitational Training Program for Advanced Japanese Research Institutes, Perspective of Plant Science
    • Place of Presentation
      Okazaki Conference Center, Okazaki, Japan
    • Year and Date
      2010-03-27
  • [Presentation] Mechanisms underlying endoplasmic reticulum body formation in Arabidopsis2009

    • Author(s)
      K. Yamada
    • Organizer
      The 1st NIBB-MPIZ Joint Symposium, Max Planck Institute for Plant Breeding Research
    • Place of Presentation
      Cologne, Germany
    • Year and Date
      20091025-26
  • [Presentation] シロイヌナズナにおける小胞体由来のオルガネラERボディの形成機構2009

    • Author(s)
      山田健志, 永野惇, 西村いくこ, 西村幹夫
    • Organizer
      第82回日本生化学会大会(シンポジウム)変貌するオルガネラ像-ダイナミクスと高次機能-
    • Place of Presentation
      神戸ポートアイランド, 神戸
    • Year and Date
      20091021-24
  • [Presentation] シロイヌナズナの小胞体由来オルガネラ“ERボディ”の形成に関する解析2009

    • Author(s)
      山田健志
    • Organizer
      第4回NIBBバイオイメージングフォーラム
    • Place of Presentation
      基礎生物学研究所, 岡崎
    • Year and Date
      20091001-02
  • [Remarks]

    • URL

      http://www.nibb.ac.jp/celmech/jp/

  • [Patent(Industrial Property Rights)] Gene Capable of Forming ER body, and Use Thereof.2010

    • Inventor(s)
      K. Yamada, I. Nishimura, M. Nishimura
    • Industrial Property Rights Holder
      National Institute for Basic Biology, Kyoto University
    • Industrial Property Number
      発明特許, PCT/JP2005/065711
    • Filing Date
      2010-09-13
    • Overseas
  • [Patent(Industrial Property Rights)] ERボディ形成能を有する遺伝子およびその利用2010

    • Inventor(s)
      山田健志, 西村いくこ, 西村幹夫
    • Industrial Property Rights Holder
      基礎生物学研究所, 京都大学
    • Industrial Property Number
      発明特許, 特願2010-68055
    • Filing Date
      2010-03-24

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Published: 2013-07-31  

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