2011 Fiscal Year Final Research Report
Unraveling regulatory network in wood formation
| Project/Area Number |
21770064
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
MITSUDA Nobutaka 独立行政法人産業技術総合研究所, 生物プロセス研究部門, 研究員 (80450667)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 植物 / ゲノム / 発現制御 / 遺伝子 / バイオマス |
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| Research Abstract |
New efficient improved yeast one-/two-hybrid screening system has been developed in which the artificial library containing only Arabidopsis genes for transcription factor(TF) is employed. This new system lets me identify TF binding to specific DNA or protein in very efficient manner. I screened TF binding to the regulatory region of genes involving to wood formation and identified many TFs as candidates. This result gradually makes me to draw entire picture of transcriptional regulatory network. Furthermore, I found some TFs rescuing phenotype of wood-less mutant in Arabidopsis and am analyzing their biological functions.
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[Journal Article] A NAC domain protein family contributing to the regulation of wood formation in poplar2011
Author(s)
Ohtani, M., Nishikubo, N., Xu B., Yamaguchi, M., Mitsuda, N., and Goue, N., Shi, F., Ohme-Takagi, M., and Demura, T.
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Journal Title
Plant J
Volume: 67
Pages: 499-512
DOI
Peer Reviewed
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[Journal Article] VASCULAR-RELATED NAC-DOMAIN7 directly regulates the expression of a broad range of genes for xylem vessel formation2011
Author(s)
Yamaguchi, M., Mitsuda, N., Ohtani, M., Ohme-Takagi, M., Kato, K., and Demura, T
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Journal Title
Plant J
Volume: 66
Pages: 579-590
DOI
Peer Reviewed
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[Journal Article] VND-INTERACTING2, a NAC domain transcription factor, negatively regulates xylem vessel formation in Arabidopsis2010
Author(s)
Yamaguchi, M., Ohtani, M., Mitsuda, N., Kubo, M., Ohme-Takagi, M., Fukuda, H., and Demura, T.
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Journal Title
Plant Cell
Volume: 22
Pages: 1249-1263
DOI
Peer Reviewed
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[Journal Article] Efficient yeast one-/two-hybrid screening using a library composed only of transcription factors in Arabidopsis thaliana2010
Author(s)
Mitsuda, N., Ikeda, M., Takada, S., Takiguchi, Y., Kondou, Y., Yoshizumi, T., Fujita, M., Shinozaki, K., Matsui, M., and Ohme-Takagi, M.
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Journal Title
Plant Cell Physiol
Volume: 51
Pages: 2145-2151
DOI
Peer Reviewed
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