2010 Fiscal Year Final Research Report
Analysis of renal function with isolated pronephros of Xenopus frog.
| Project/Area Number |
21770069
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Morphology/Structure
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| Research Institution | University of Toyama |
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Principal Investigator |
KONNO Norifumi University of Toyama, 大学院・理工学研究部, 助教 (50507051)
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| Project Period (FY) |
2009 – 2010
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| Keywords | ツメガエル / 発生 / 腎臓 / 体液調節 / 比較生理学 |
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| Research Abstract |
To understand integrative renal functions in vertebrates, we explored and characterized genes expressing in pronephros of amphibian tadpole and mesonephros of teleost. We constructed the GFP expression vector linked 5'-upstream promoter of Na^+, K^+-ATPase, marker protein of renal tubule, and succeeded in isolating a living pronephric tubule by using transgenic technique. Furthermore, we explored genes associated with polycystic kidney disease (PKD) by a PCR-based subtractive hybridization method using pc mutant, a PKD model of medaka. As a result, we identified 2 genes (vasotocin type-2 receptor and aquaporin-8) reduced markedly in the kidney of pc mutant. Currently, we explore genes expressing in pronephros by global analysis and then characterize 2 genes found as candidate gene associated with teleost PKD.
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[Journal Article] Isolation and characterization of two cDNAs encoding the neuromedin U receptor from goldfish brain.2011
Author(s)
Maruyama, K., Kaiya, H., Miyazato, M., Konno, N., Wakasugi, T., Uchiyama, M., Shioda, S., Murakami, N., Matsuda, K.
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Journal Title
Journal of Neuroendocrinology 23
Pages: 282-291
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[Journal Article] Molecular cloning and characterization of V2-type receptor in two ray-finned fish, gray bichir2010
Author(s)
Konno, N., Kurosawa, M., Kaiya, H., Miyazato, M., Matsuda, K., Uchiyama, M.
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Journal Title
Polypterus senegalus and medaka, Oryzias latipes Peptides 31
Pages: 1273-1279
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