Structural and functional analysis of Uro-adherence factor A(UafA) from Staphylococcus saprophyticus

2011 Fiscal Year Final Research Report

• Project/Area Number: 21770101
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Structural biochemistry
• Research Institution: Hokkaido University
• Principal Investigator: TANAKA Yoshikazu 北海道大学, 創成研究機構, 特任助教 (20374225)
• Project Period (FY): 2009 – 2011
• Keywords: 接着因子 / 結晶構造解析 / 腐性ブドウ球菌

Research Abstract

Pathogenic bacteria express several cell wall-anchored proteins to adhere to the surface of host cell, whereas Staphylococcus saprophyticus, a uropathogen involved in urinary tract infection of young females, possesses a unique cell wall-anchored protein, UafA. In this study, we investigated the adhesion mechanism of UafA by structure analysis and biochemical assay.

Research Products

• [Journal Article] Crystal Structure of the Octameric Pore of Staphylococcalγ-hemolysin Reveals theβ-barrel Pore Formation Mechanism by Two Components (2011)
  • Author(s): Keitaro Yamashita, Yuka Kawai, Yoshikazu Tanaka, Nagisa Hirano, Jun Kaneko, Noriko Tomita, Makoto Ohta, Yoshiyuki Kamio, Min Yao, and Isao Tanaka
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 108 Pages: 17314-17319
  • Peer Reviewed
• [Journal Article] Molecular basis of dihydrouridine formation on tRNA (2011)
  • Author(s): Futao Yu, Yoshikazu Tanaka, Keitaro Yamashita, Takeo Suzuki, Akiyoshi Nakamura, Nagisa Hirano, Tsutomu Suzuki, Min Yao, and Isao Tanaka
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 108 Pages: 19593-19598
  • Peer Reviewed
• [Journal Article] Crystal structure of the functional region of Uro-adherence factor A(UafA) from Staphylococcus saprophyticus reveals participation of the B domain in ligand binding (2011)
  • Author(s): Eriko Matsuoka, Yoshikazu Tanaka, Makoto Kuroda, Yuko Shouji, Toshiko Ohta, Isao Tanaka, and Min Yao
  • Journal Title: Protein Science Volume: 20 Pages: 406-416
  • Peer Reviewed
• [Journal Article] Structural and Mutational Analyses of the Receptor Binding Domain of Botulinum D/C Mosaic Neurotoxin : Insight into the Ganglioside Binding Mechanism (2011)
  • Author(s): Nuemket Nipawan, Yoshikazu Tanaka, Kentaro Tsukamoto, Takao Tsuji, Keiji Nakamura, Shunji Kozaki, Min Yao, and Isao Tanaka
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 411 Pages: 433-439
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray crystallographic analysis of dihydrouridine synthase from Thermus thermophilus and its complex with tRNA (2011)
  • Author(s): Futao Yu, Yoshikazu Tanaka, Shiho Yamamoto, Akiyoshi Nakamura, Shunsuke Kita, Nagisa Hirano, Isao Tanaka, and Min Yao
  • Journal Title: Acta Crystallographica Section F Volume: F67 Pages: 685-688
  • Peer Reviewed
• [Journal Article] 2-Methyl-2, 4-pentanediol induces spontaneous assembly of staphylococcal alpha-hemolysin into heptameric pore structure (2011)
  • Author(s): Yoshikazu Tanaka, Nagisa Hirano, Jun Kaneko, Yoshiyuki Kamio, Min Yao, and Isao Tanaka
  • Journal Title: Potein Science Volume: 20 Pages: 448-456
  • Peer Reviewed
• [Presentation] Crystal structure of pore-forming toxin from Staphylococcus aureus-An example of the utility of crystal structure analysis- (2012)
  • Author(s): Yoshikazu Tanaka, Keitaro Yamashita, Yuka Kawai, Jun Kaneko, Noriko Tomita, Makato Ohta, Yoshiyuki Kamio, Min Yao, and Isao Tanaka
  • Organizer: Japan-Australia Symposium on Plant Sciences for Agriculture(JAS2012)
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2012-01-20
• [Presentation] Crystal Structure of the Octameric Pore of Staphylococcal gamma-hemolysin (2011)
  • Author(s): Yoshikazu Tanaka, Keitaro Yamashita, Yuka Kawai, Nagisa Hirano, Jun Kaneko, Noriko Tomita, Makato Ohta, Yoshiyuki Kamio, Min Yao, and Isao Tanaka
  • Organizer: The Eighth International Conference on Flow Dynamics(ICFD2011)
  • Place of Presentation: ホテルメトロポリタン仙台(仙台市)
  • Year and Date: 2011-11-10
• [Presentation] 黄色ブドウ球菌由来巨大蛋白質Ebhの全体構造に関する研究 (2011)
  • Author(s): 田中良和
  • Organizer: 日本化学会東北支部化学系学協会東北大会
  • Place of Presentation: 東北大学(仙台市)
  • Year and Date: 2011-09-17
• [Presentation] Crystal structure of dihydrouridine synthase in complex with RNA reveals its tRNA recognition and reaction mechanisms (2011)
  • Author(s): Yoshikazu Tanaka, Futao Yu, Keitaro Yamashita, Akiyoshi Nakamura, Takeo Suzuki, Tsutomu Suzuki, Min Yao, and Isao Tanaka
  • Organizer: The 16th Annual Meeting of the RNA Society(Kyoto)
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2011-06-17
• [Presentation] RNA binding mechanism of ThiI deduced from structural and binding analyses of a minimal RNA ligand (2010)
  • Author(s): Yoshikazu Tanaka, Shiori Yamagata, Yu Kitago, Yoko Yamada, Sarin Chimnaronk, Min Yao and Isao Tanaka
  • Organizer: Asian Crystallographic Association
  • Place of Presentation: Busan, Korea
  • Year and Date: 20101031-1103
• [Presentation] 部分RNAの結晶構造解析から推察されたtRNAチオ化修飾酵素ThiIのtRNA認識機構 (2010)
  • Author(s): 田中良和
  • Organizer: 九州シンポジウム
  • Place of Presentation: 玄海ロイヤルホテル(宗像市)
  • Year and Date: 20100909-11
• [Remarks]
  • URL: http://altair.sci.hokudai.ac.jp/g6/