2010 Fiscal Year Final Research Report
Structure basis for ligand recognition and singnal inducing mechanism involved in TLR4・MD-2 complex.
| Project/Area Number |
21770104
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Structural biochemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
TOMA Sachiko The University of Tokyo, 大学院・薬学系研究科, 助教 (40363535)
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| Project Period (FY) |
2009 – 2010
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| Keywords | X線結晶解析 |
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| Research Abstract |
In this project, I tried to determine the crystal structure of TLR4-MD-2 complex. TLR4, which is a membrane protein, is a member of pattern recognition proteins. TLR4-MD-2 complex recognizes LPS known as endotoxin. TLR4-MD-2 complex could be expressed on plasma membrane in sf-9 insect cell. The total expression level on the membrane was sufficient for using crystallization. However, almost all expressed TLR4-MD-2 could not be extracted from the membrane. From small scale purification experiment, 60% purity TLR4-MD-2 complex solution was obtained.
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