2010 Fiscal Year Final Research Report

Functional analysis of E. coli Hsp90 (HtpG) to ribosome biogenesis

Research Project

Project/Area Number	21770107
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Structural biochemistry
Research Institution	Tokyo Institute of Technology
Principal Investigator	MOTOJIMA Fumihiro Tokyo Institute of Technology, 総合生命科学部, 助教 (70372464)
Project Period (FY)	2009 – 2010
Keywords	変性とフォールディング / 分子シャペロン
Research Abstract	We found that a 50S ribosomal protein L2 was purified as a complex with His-tagged HtpG from Escherichia coli cultured in minimum medium at 45oC. L2 specifically activated ATPase of HtpG more than ten-fold. HtpG was also found in fractions of 30S and 50S ribosome subunits in vivo. These results suggested that HtpG associates with various ribosomal proteins including L2 as a client protein and contributes for ribosome biogenesis.

(3 results)