2010 Fiscal Year Final Research Report
Structural analysis of the ribosome-bound GTPase family
| Project/Area Number |
21770108
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Structural biochemistry
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| Research Institution | Niigata University |
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Principal Investigator |
ITO Kosuke Niigata University, 自然科学系, 助教 (20502397)
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| Project Period (FY) |
2009 – 2010
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| Keywords | リボソーム / GTPaseセンター / GTPaseファミリー / 超好熱古細菌 / X線結晶構造解析 |
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| Research Abstract |
In translation process, GTP-bound translation factors bind to "GTPase-associated center" in the ribosomal large subunit one by one, and cause GTP hydrolysis. To elucidate its hydrolysis mechanism, the structure determinations of the complex structures of GTP-bound translation factors/GTPase-associated center are necessary. Recently, our research group has detected the direct binding between EF-1α, one of the GTP-bound translation factor, and P1 protein, a component of GTPase-associated center. In this research, we have determined the complex structure of EF-1α and P1 protein by X-ray crystallography in an atomic resolution.
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