2010 Fiscal Year Final Research Report
NMR structural analysis for the whole structure of human general transcription factor, TFIIE.
| Project/Area Number |
21770121
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Structural biochemistry
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| Research Institution | Yokohama City University |
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Principal Investigator |
OKUDA Masahiko Yokohama City University, 生命ナノシステム科学研究科, 特任助教 (60448686)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 構造生物学 / 転写 |
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| Research Abstract |
This study was the NMR structural analysis for the whole structure of human TFIIE, a member of the general transcription factors, needed for the initiation of transcription for protein coding genes. Human TFIIE is a heterodimer, consisting of an alpha and a beta subunit. Although the tendency to aggregate besides the high molecular weight (~80kDa) made the structural analysis difficult, full use of the latest NMR technologies enabled to gain structural information on three functional domains, identify widely existing intrinsically disordered regions and reveal the dynamics.
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