2010 Fiscal Year Final Research Report
Three-dimensional structure of lipid-raft localized membrane protein and elucidation of the mechanism forming membrane vesicles
| Project/Area Number |
21770122
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Structural biochemistry
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| Research Institution | University of Shizuoka |
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Principal Investigator |
YOKOYAMA Hideshi University of Shizuoka, 薬学部, 講師 (70433208)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 膜小胞 / ストマチン / フロチリン / 脂質ラフト / 結晶化 / X線結晶構造解析 |
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| Research Abstract |
Stomatin and flotillin, which are involved in forming membrane vesicles, are located in lipid rafts. Until now, it is not elucidated how these proteins function. I tried to prepare proteins, human stomatin and flotillin using Escherichia coli, and to determine three-dimensional structures in order to elucidate their functions. I succeeded to get these proteins. And if I can get crystals of these proteins, I will determine the structures.
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