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2010 Fiscal Year Final Research Report

Dynamic behavior analysis of membrane molecules in glycolipid enriched membrane microdomain

Research Project

  • PDF
Project/Area Number 21770124
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Structural biochemistry
Research InstitutionTokai University

Principal Investigator

KABAYAMA Kazuya  Tokai University, 糖鎖科学研究所, 准教授 (00399974)

Project Period (FY) 2009 – 2010
Keywords糖脂質 / 糖鎖 / マイクロドメイン / 膜受容体 / イメージング
Research Abstract

Membrane lateral heterogeneity is accepted as a requirement for the function of biological membranes, and the notion of "raft/microdomain" gives specificity to this concept. In this study, measurement of the lateral diffusion of IR was performed by fitting analysis to fluorescence recovery curves and trace analysis to individual fluorescent spots, which provided diffusion coefficient (D). It shows us how fast membrane molecules are diffusing at the change of membrane environment such as before and after stimulation by cholesterol depression. We will utilize these techniques for the lateral diffusion analysis of membrane receptor in another assay conditions, such as treatment of glycosphingolipid (GSL) inhibitor, use of GSL deficient cells or of pathologic samples.

  • Research Products

    (16 results)

All 2011 2010 Other

All Journal Article (2 results) Presentation (13 results) Remarks (1 results)

  • [Journal Article] 糖脂質マイクロドメインの機能および構造解析2011

    • Author(s)
      樺山一哉, 鈴木佑典
    • Journal Title

      東海大学糖鎖科学研究所紀要(東海大学糖鎖科学研究所) Vol.5

      Pages: 26-32

  • [Journal Article] 糖脂質マイクロドメインと膜受容体の相互作用解析2010

    • Author(s)
      樺山一哉
    • Journal Title

      PEPTIDE NEWSLETTER JAPAN.(日本ペプチド学会) No.78

  • [Presentation] Modulation of Growth Factor Receptors in Membrane Microdomains2011

    • Author(s)
      Kazuya Kabayama
    • Organizer
      第131回日本薬学会年会特別シンポジウム(震災のため中止*要旨あり)
    • Place of Presentation
      静岡
    • Year and Date
      20110328-20110331
  • [Presentation] Analysis of glycolipid-membrane protein interaction by optical microscope2010

    • Author(s)
      Kazuya Kabayama
    • Organizer
      第二回光塾
    • Place of Presentation
      大阪
    • Year and Date
      20101211-20101212
  • [Presentation] 2型糖尿病治療薬としての新規グルコシルセラミド生合成酵素阻害剤の開発2010

    • Author(s)
      横井麻加, 出口友紀子, 郷慎司, 樺山一哉, 吉村祐一, 高畑廣紀, 井ノ口仁一
    • Organizer
      BMB2010
    • Place of Presentation
      神戸
    • Year and Date
      20101207-20101210
  • [Presentation] Measurement of the lateral diffusion of insulin receptor in membrane microdomain by fluorescence recovery after photobleaching2010

    • Author(s)
      Kazutaka Oda, Kazuya Kabayama
    • Organizer
      BMB2010
    • Place of Presentation
      神戸
    • Year and Date
      20101207-20101210
  • [Presentation] Convenient and rapid removal of detergent from gangliosides in detergent-resistant membrane microdomains2010

    • Author(s)
      Yusuke Suzuki, Kazuya Kabayama
    • Organizer
      BMB2010
    • Place of Presentation
      神戸
    • Year and Date
      20101207-20101210
  • [Presentation] Alteration of lipid component in microdomain affects lateral diffusion of membrane receptor2010

    • Author(s)
      Kazuya Kabayama
    • Organizer
      BMB2010
    • Place of Presentation
      神戸
    • Year and Date
      20101207-20101210
  • [Presentation] Measurement of the lateral diffusion of growth factor receptors in membrane microdomain by live cell imaging system2010

    • Author(s)
      Kazuya Kabayama
    • Organizer
      The 2nd ACGG Conference
    • Place of Presentation
      台北
    • Year and Date
      20101026-20101030
  • [Presentation] Glycolipid-enriched microdomain control lateral diffusion of membrane receptors2010

    • Author(s)
      Kazuya Kabayama
    • Organizer
      第48回日本生物物理学会年会シンポジウム
    • Place of Presentation
      仙台
    • Year and Date
      20100920-20100922
  • [Presentation] New approaches to detect behavior of growth factor receptors in plasma membrane microdomains2010

    • Author(s)
      Kazuya Kabayama, Jin-ichi Inokuchi
    • Organizer
      第25回国際糖質シンポジウム(ICS2010)
    • Place of Presentation
      幕張
    • Year and Date
      20100801-20100806
  • [Presentation] Dynamic behavior analysis of insulin signaling molecules in glycolipid enriched membrane microdomain2010

    • Author(s)
      Kazuya Kabayama, Jin-ichi Inokuchi
    • Organizer
      the 27th Naito Conference on "Membrane Dynamics and Lipid Biology [I]"(口頭発表)
    • Place of Presentation
      札幌
    • Year and Date
      20100629-20100702
  • [Presentation] 糖脂質マイクロドメインの機能および構造解析2010

    • Author(s)
      樺山一哉
    • Organizer
      第8回糖鎖科学コンソーシアムシンポジウム
    • Place of Presentation
      品川
    • Year and Date
      2010-11-29
  • [Presentation] 細胞膜上における膜受容体の挙動解析2010

    • Author(s)
      尾田和隆, 鈴木優里, 樺山一哉
    • Organizer
      東海大・成蹊大・群馬大合同セミナー
    • Place of Presentation
      東海大学山中湖セミナーハウス
    • Year and Date
      2010-10-24
  • [Presentation] ラスター画像相関分光法による膜分子の動態解析2010

    • Author(s)
      樺山一哉
    • Organizer
      第2回総合医学研究所・糖鎖科学研究所合同シンポジウム(招待講演)
    • Place of Presentation
      東海大学
    • Year and Date
      2010-05-28
  • [Remarks] ホームページ等

    • URL

      http://www.tsc.u-tokai.ac.jp/pubhome/glyco/

URL: 

Published: 2012-02-13   Modified: 2016-04-21  

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