2010 Fiscal Year Final Research Report
Dynamic behavior analysis of membrane molecules in glycolipid enriched membrane microdomain
| Project/Area Number |
21770124
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Structural biochemistry
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| Research Institution | Tokai University |
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Principal Investigator |
KABAYAMA Kazuya Tokai University, 糖鎖科学研究所, 准教授 (00399974)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 糖脂質 / 糖鎖 / マイクロドメイン / 膜受容体 / イメージング |
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| Research Abstract |
Membrane lateral heterogeneity is accepted as a requirement for the function of biological membranes, and the notion of "raft/microdomain" gives specificity to this concept. In this study, measurement of the lateral diffusion of IR was performed by fitting analysis to fluorescence recovery curves and trace analysis to individual fluorescent spots, which provided diffusion coefficient (D). It shows us how fast membrane molecules are diffusing at the change of membrane environment such as before and after stimulation by cholesterol depression. We will utilize these techniques for the lateral diffusion analysis of membrane receptor in another assay conditions, such as treatment of glycosphingolipid (GSL) inhibitor, use of GSL deficient cells or of pathologic samples.
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