Elucidation of dynamics and function of an iPS cell reprogramming factor Sox2 from the view point of an intrinsically disordered protein

2010 Fiscal Year Final Research Report

• Project/Area Number: 21770126
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Structural biochemistry
• Research Institution: 公益財団法人サントリー生命科学財団 (2010); Suntory Institute for Bioorganic Research (2009)
• Principal Investigator: SUGASE Kenji 公益財団法人サントリー生命科学財団, 生物有機科学研究所, 主席研究員 (00300822)
• Project Period (FY): 2009 – 2010
• Keywords: 分子認識及び相互作用

Research Abstract

NMR relaxation dispersion studies revealed that Sox2 fluctuates when it is free in solution, in particular the N-terminal helix α1 and the regions contacting the helixα1 are unfolded with a population of 2%. In contrast, no relaxation dispersion was observed for Sox2 in the bound form, indicating that Sox is stable in the bound form on μs-ms timescales. Since the helix α1 binds directly to DNA, the fluctuation observed for the free form is supposed to be important for non-specific binding to DNA.

Research Products

• [Journal Article] Calcitonin in a protochordate, Ciona intestinalis--the prototype of the vertebrate calcitonin/calcitonin gene-related peptide superfamily. (2009)
  • Author(s): Sekiguchi T, Suzuki N, Fujiwara N, Aoyama M, Kawada T, Sugase K, Murata Y, Sasayama Y, Ogasawara M, Satake H
  • Journal Title: FEBS J. Vol.276 Pages: 4437-4447
  • Peer Reviewed
• [Journal Article] Elucidating slow binding kinetics of a protein without observable bound resonances by longitudinal relaxation NMR spectroscopy
  • Author(s): Sugase K
  • Journal Title: J.Biomol.NMR in press
  • Peer Reviewed
• [Journal Article] Lipopolysaccharide induces raft domain expansion in membrane composed of a phospholipid-cholesterol-sphingomyelin ternary system.
  • Author(s): Nomura K, Maeda M, Sugase K, Kusumoto S
  • Journal Title: Innate Immun. in press
  • Peer Reviewed
• [Presentation] Fluctuation and function of nuclear proteins (2010)
  • Author(s): 菅瀬謙治
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2010-09-20
• [Presentation] Fitting Relaxation Dispersion Data With A New Software Package GLOVE (2010)
  • Author(s): 菅瀬謙治
  • Organizer: XXIVth International Conference on Magnetic Resonance in Biological Systems
  • Place of Presentation: Cairns, Australia
  • Year and Date: 2010-08-24
• [Presentation] NMR による天然変性タンパク質の動的構造解析 (2010)
  • Author(s): 菅瀬謙治
  • Organizer: 第10回日本蛋白質科学会年会
  • Place of Presentation: 札幌
  • Year and Date: 2010-06-18
• [Presentation] 動きの面から見た核内タンパク質の機能 (2010)
  • Author(s): 菅瀬謙治
  • Organizer: ターゲットタンパク研究プログラム第3回研究交流会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-04
• [Presentation] 天然変性状態と機能の相関 (2009)
  • Author(s): 菅瀬謙治
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-11
• [Presentation] Elucidation of protein-protein interactions by relaxation NMR spectroscopy (2009)
  • Author(s): 菅瀬謙治
  • Organizer: The 3rd APNMR Biannual Asia-Pacific NMR Symposium
  • Place of Presentation: Jeju, Korea
  • Year and Date: 2009-10-27
• [Presentation] 天然変性タンパク質の構造と機能の最前線 (2009)
  • Author(s): 菅瀬謙治
  • Organizer: 第49回生命科学夏の学校
  • Place of Presentation: 三田
  • Year and Date: 2009-08-30
• [Book] Water and Biomolecules : Physical Chemistry of Life Phenomena (Biological and Medical Physics, Biomedical Engineering) (ed. Kuwajima K, Goto Y, Hirata F, Terazima M, Kataoka M) (2009)
  • Author(s): Wright PE, Felitsky DJ, Sugase K, Dyson HJ
  • Total Pages: 307
• [Remarks] ホームページ等