2011 Fiscal Year Final Research Report
Structural and biochemical analysis of Sec translocon machinery
| Project/Area Number |
21770135
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional biochemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Keywords | タンパク質 / 膜透過 / 膜タンパク質 / 膜組込 / トランスロコン / Sec / 構造解析 / 構造生物学 |
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| Research Abstract |
Transport of proteins across membranes is one of the fundamental and essential cellular activities in all organisms. We solved the first 3D structure of a membrane component SecDF, which plays an important role in efficient translocation of newly synthesized proteins across the membrane in bacteria. From the structural features and functional analyses, we proposed that SecDF functions as a membrane-integrated chaperone, which drives movement of proteins without using a major currency, ATP, of energy, but with remarkable cycles of conformational changes, powered by a proton H+ion) gradient across the membrane. Our model of the SecDF function has been verified by a series of biochemical and biophysical approaches. This research uncovers a new, atomic-level principle that underlies the cellular protein delivery systems in particular and biological mechanisms of active transport in general, by which cells acquire or dispose not only macromolecules but also ions, drugs and nutrients.
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Author(s)
Kato, H. E., Zhang, F., Yizhar, O., Ramakrishnan, C., Nishizawa, T., Hirata, K., Ito, J., Aita, Y., Tsukazaki, T., Hayashi, S., Hegemann, P., Maturana, A. D., Ishitani, R., Deisseroth, K., and Nureki O
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Journal Title
Nature
Volume: 482
Pages: 369-374
DOI
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[Journal Article] Structure and function of a membrane component SecDF that enhances protein export2011
Author(s)
Tsukazaki, T., Mori, H, Echizen, Y., Ishitani, R., Fukai, S., Tanaka, T., Perederina A., Vassylyev, D. G., Kohno, T., Maturana, A. D., Ito, K., and Nureki, O
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Journal Title
Nature
Volume: 474
Pages: 235-238
DOI
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[Journal Article] Crystallization and preliminary X-ray diffraction of the first periplasmic domain of SecDF, a translocon-associated membrane protein, from Thermus thermophilus2011
Author(s)
Echizen, Y., Tsukazaki, T., Dohmae, N., Ishitani, R. and Nureki, O
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Journal Title
Acta. Crystallograph. Sect. F Struct. Biol. Cryst. Commun
Volume: 67
Pages: 1367-1370
URL
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[Journal Article] Mg^<2+>-dependent gating ofbacterial MgtE channel underlies Mg^<2+> homeostasis2009
Author(s)
Hattori, M., Iwase, N., Furuya, N., Tanaka, Y., Tsukazaki, T., Ishitani, R., Maguire, M. E., Ito, K. and Nureki, O
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Journal Title
EMBO J
Volume: 28
Pages: 3602-361
URL
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